摘要
目的：了解新疆两城市丙型肝炎病毒感染者中存在哪些基因型，以便为今后防治ＨＣＶ感染提供依据。方法：用ＲＴ－ＰＣＲ技术，选择ＨＣＶＮＳ－５区设计的分型引物（１ａ、１ｂ、２ａ和２ｂ）进行基因分型。结果：我们检测了１２８例（乌鲁木齐市９７例，克拉玛依市３１例）已证实为丙型肝炎病毒感染者的血清，其中基因分型检测出的９８例中可分为１ａ型１４例（１４．３％）、１ｂ型５８例（５９．３％）、２ａ型１１例（１１．８％）、２ｂ型１０例（１０．３％），混合感染５例（５．２％）（１ａ＋１ｂ型４例，１ｂ＋２ａ型１例）。在乌鲁木齐市和克拉玛依市的ＨＣＶ感染者中１ａ型分别为１１／７３例和３／２５例，１ｂ型分别为４６／７３例和１２／２５例。结论：表明新疆至少存在ＨＣＶ１ａ、１ｂ、２ａ和２ｂ基因型，但以１ｂ基因型感染为主。比较乌鲁木齐市和克拉玛依市两城市的ＨＣＶ基因型分布显示１ａ型和１ｂ型分布有明显差别（χ２＝３６，Ｐ＜０．００１）。另３０例用本方法未能检出分型。
Objective: It is designed to analyse which HCV genotypes are more prevalent in Xinjiang in order to prevent and treat HCV infection approximately. Methods: We detected HCV genotypes of serum from the patients with post blood transfusion HCV infection in Urumqi and Kelamayi cities of Xinjiang, with RT PCR and type specific primer assay (1a,1b,2a and 2b) based on the HCV gene sequence of non structural region 5 (NS 5). Results: The results of the study showed that 98 out of 128 serum samples from the patients with HCV infection were genotype positive. The genotypes in two cities were 1a (14), 1b(58), 2a(11), 2b(10) and mixed infection (5) respectively. The genotypes 1a and 1b were 11/73(15.1%) and 46/73(63.2%) in Urumqi city, while 3/25(12.1%) and 12/25(48.5%) in Kelamayi city. The mixed infection of genotypes as 1a+1b and 1b+2a were 2/73(2.8%) and 0/73 in Urumqi city, and 2/25(8%) and 1/25(4%) in Kelamayi respectively. Other 30 serum samples couldn't be detected by this assay. Conclusions: This study showed that there were at least four HCV genotypes 1a, 1b, 2a and 2b in Xinjiang, but the prevalent genotype was 1b. Comparison of the genotypes 1a and 1b in Urumqi with in Kelamayi indicated that the distribution of HCV genotypes 1a and 1b was quite different(χ 2=36, P< 0.001 ), and the mixed genotype infection in two cities was different, too. The genotypes of 30 samples from the patients with HCV infection could not be detected indicating that other genotypes of HCV may exist in Xinjiang.
