摘要
根据猪MyoG基因的部分序列和绵羊MyoG mRNA设计引物,以成都麻羊、金堂黑山羊、白玉黑山羊和高原型藏山羊基因组为模板,应用PCR技术克隆测定MyoG基因序列.序列分析结果表明,成都麻羊MyoG基因长1957bp,由3个外显子和2个内含子组成,其中外显子Ⅰ、Ⅱ、Ⅲ的大小分别为398bp、82bp、122bp,内含子Ⅰ和内含子Ⅱ的大小分别为765bp和589bp.成都麻羊MyoG基因序列与金堂黑山羊、白玉黑山羊和高原型藏山羊的MyoG基因序列进行比对,同源性分别为99.9%、99.7%和99.3%,而它们氨基酸序列的同源性都为100%.这为进一步研究成都麻羊MyoG基因的表达、生物学活性和应用奠定了一定基础.
According to partial secquence of pig MyoG gene and sheep MyoG mRNA, primers are designed. On the template of genome of Chengdti Grey .Goats, Jintang Black Goat, Baiyu Black Goat, Tibetan Goat,MyoG gene is cloned by PCR. The （esults of secque（ce analyzing provide that Myo.G gene of hengdu Grey Goat consists of 3 exons and 2 introns, and the length of exon Ⅰ,exon Ⅱ,exon Ⅲ intron Ⅰand intron Ⅱ is 398bp,82bp,122bp,765bp and 589bp.Myog gene secquence of Chengdu Grey Goat is compared with that of jintang black Goat,Baiyu Black Goat,Tibetan Goat,and the result shows that the overall similarity is 99.9%,99.7%and 99.3% correspondingly,yet their similarity of amino acid secquence is 100% coincidently.This resarch provides a major base for researching the expression and biologic activity of Chengdu Grey Goat MyoG gene.
出处
《西南民族大学学报（自然科学版）》
CAS
2009年第1期84-88,共5页
Journal of Southwest Minzu University(Natural Science Edition)
基金
四川省应用基础项目(2008LY0067-1)
