摘要
目的:观察消癥散对荷H22肝癌实体瘤小鼠肿瘤生长的抑制作用和对腹水瘤小鼠生存期的延长作用,探讨消癥散抗肿瘤的作用机制。方法:①制作小鼠H22肝癌实体瘤模型,分为模型组,消癥散低、中、高剂量组,5-FU组,5-FU+消癥散中剂量组。治疗10d后,计算抑瘤率,检测血清白介素-2(IL-2)含量,观察肿瘤组织病理切片,免疫组化法观察细胞凋亡相关基因-2(Bcl-2)表达水平。②制作小鼠H22肝癌腹水瘤模型,分组同上,记录各组小鼠生存天数,计算生命延长率。结果:消癥散能够抑制肿瘤的生长,并呈现一定的剂量相关性;消癥散各剂量组肿瘤组织镜下病理示肿瘤细胞出现核固缩、片状坏死、出血及周边炎症细胞浸润等;消癥散能增强荷瘤小鼠IL-2活性,各剂量降低Bcl-2表达水平;消癥散与抗癌西药5-FU配合使用能够延长腹水瘤小鼠的生存期。结论:消癥散具有抑制肿瘤生长,增强机体的抗肿瘤能力,诱导肿瘤细胞凋亡,降低肿瘤的持续增殖能力等作用。消癥散与抗癌西药合用能够延长腹水瘤小鼠的生存期。
Objective： To observe the effect of prolonging life and inhibiting tumor growth of Xiaozhensan on H22 cells-bearing mice and study the mechanism of action. Methods ： ①H22 cells-bearing mice models were made by subcutaneous injection and were divided into six groups： model group, low, middle and high dosage groups of Xiaozhensan, 5-FU group, 5-FU plus Xiaozhensan middle dosage group. After 10 days＇ treatment, the mice were killed and the anti-tumor rates were calculated, to detect the content of IL-2 and observe pathological sections of tumor and expression of Bcl-2 by immunohistochemical method. ②H22 cells-bearing ascites mice models were divided as the above. The living days of the mice were recorded, the prolongation of life span were calculated. Results： Xiaozhensan had the function of inhibiting the growth of tumor, and there was the dependability between quantity and effect. In pathomorphology, the sections of Xiaozhensan groups showed nuclear shrinkage, necrosis, hemorrhage, inflammatory cell infiltration on the periphery, etc. Xiaozhensan could increase the content of IL-2 and inhibit the expression of Bcl-2. Xiaozhensan combined with 5-FU can prolong the life span of the mice bearing ascites tumor. Conclusion： Xiaozhensan had the function of inhibiting the growth of tumor. The mechanism of action may be concerned with these aspects： enhancing the cellullar immunologic response and strengthening the ability to resist tumor, inducing apoptosis and weakening reproductive activity of tumor, etc. Middle dosage group of Xiao Zhen San combined with anticarcinogenic western medicin can prolong the life span of the mice hearing ascites tumor. This study provided dependable experimental documents for clinical application of Xiaozhensan, and had some guiding significance in clinic.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2009年第2期202-204,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
浙江省中管局课题(No.2006C020)
