摘要
目的 研究铜绿假单胞菌连续分离株对氯霉素与四环素相关耐药基因的分布情况。方法 以K-B法检测铜绿假单胞菌对17种抗菌药物的耐药性；以PCR法检测20株铜绿假单胞菌对氯霉素耐药相关基因catB、cmlA，四环素耐药相关基因tetA、tetB以及小多药外排泵基因smr-2，以PCR直接全自动荧光法进行cmlA基因测序。结果20株铜绿假单胞菌对17种抗菌药物的耐药率在10．0％～100．0％，部分菌株呈现多药耐药现象；20株铜绿假单胞菌对氯霉素耐药的15株中检出cmlA6株；20株铜绿假单胞菌对四环素与米诺环素均耐药，但tet、tetB及smr-2均未检出；作cmlA阳性基因测序，测得序列经比对与美国核酸库已登录的cmlA7、cmlA8序列99．0％相同。结论铜绿假单胞菌对氯霉素耐药主要与cmlA基因相关，对四环素、米诺环素耐药与tet、tetB以及小多药外排泵基因smr2无关，存在另外的四环素相关耐药机制。
OBJECTIVE To explore the distribution of related resistance genes of chloramphenicol and tetracycline in Pseudomonas aeruqinosa isolates. METHODS The antibiotics susceptibility was tested by K-B method,catB cmlA, tetA tetB and smr-2 were detected by polymerase chain reaction （PCR）, the gene cmlA was sequenced by PCR fluorescence spectrophotometry. RESULTS The drug-resistant rates of 20 strains of P. aeruginosa against 17 kinds of antibiotics ranged from 10.0% to 100.0% ,and multidrug resistant strains were found. The gene of cmlA had been detected in 6 strains of 15 resistant P. aeruginosa isolates, but the genes of tetA,tetB and smr had not been detected. The cml was sequenced, and compared with GenBank, the result showed the gene fragment shared 99.0% homology in nucleotides with the GenBank sequences of cmlA7 and cmlAS. CONCLUSIONS Resistance to antibiotics in P. aeruginosa of our hospital is mainly related to emlA. The resistance to chloramphenicol and tetracycline was not related to tetA,tetB and smr-2.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第2期135-137,共3页
Chinese Journal of Nosocomiology
关键词
铜绿假单胞菌
氯霉素
四环素
耐药基因
Pseudomonas aeruginosa
Chloramphenicol
Tetracycline
Resistance gene
