摘要
为了确定ＣＲ２受体是否在ＥＢ病毒对Ｔ淋巴细胞的感染中起一定的作用，研究了ＣＲ２受体在Ｔ细胞的表达和ＥＢ病毒对Ｔ细胞的感染。从正常人外周血中分离的ＣＤ４＋和ＣＤ８＋Ｔ细胞其纯度达９８％以上。经逆转录多聚酶链反应（ＲＴ－ＰＣＲ）和双色荧光抗体染色并结合流式细胞分析仪分析的结果证明，在ＣＤ４＋和ＣＤ８＋Ｔ细胞检测不到ＣＲ２ｍＲＮＡ和细胞表面的ＣＲ２抗原。然而，在体外用ＥＢ病毒感染这些Ｔ细胞后，可以用ＰＣＲ方法检测到ＥＢ病毒特异性ＥＢＮＡ２ＡＤＮＡ序列和用ＲＴ－ＰＣＲ方法检测到ＥＢ病毒ＢＲＬＦ１ｍＲＮＡ表达。结果提示，ＥＢ病毒可以通过一种不同于Ｂ细胞ＣＲ２受体的、尚未确定的ＥＢ病毒受体感染ＣＤ４＋和ＣＤ８＋Ｔ细胞。这一结果为进一步研究ＥＢ病毒感染与Ｔ细胞淋巴瘤的关系提供了新的直接的线索。
EpsteinBarr virus(EBV) is a causative agent of infectious mononucleosis and strongly associated with nasopharyngeal carcinoma and endemic form of Burkitt's lymphoma. Conventional thought is that EBV only infects B cells and epithelial cells via a type 2 complement receptor(CR2, now renamed CD21), a receptor for C3d. T cells are normally considered to be negative for CR2 expression yet EBVcarrying T cell disorders are nowrecognized. To determine whether CR2 plays a role in T cell infection by EBV, the CR2 status and EBV infection of T cells, CD4+ and CD8+ subpopulation were analyzed. CD4+ and CD8+ T cells were separated into populations that were greater than 98% pure by flow cytometer from normal adult peripheral blood lymphocytes. CR2 mRNA and surface CR2 antigen could not be detected by RTPCR or antiCR2 monoclonal antibody(B2) staining. However, EBNA2A DNA and BRLF1 mRNA signals could be detected from DNA and RNA isolation of CD4+ and CD8+ T cells infected with EBV B95.8. The results suggest that an unidentified EBV receptor distinct from B cells CR2 might serves as the receptor for EBV infection. This finding will be useful in the study of EBV infection and T cells lymphoma and may lead to improve antiviral therapy in these patients.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第2期86-88,共3页
Chinese Journal of Immunology
