摘要
目的建立一种与临床更相关的人舌鳞癌动物模型。方法将人舌鳞癌Ｔｃａ８１１３细胞行裸鼠原位移植并连续传代。对每代移植瘤和裸鼠口底、颌下组织、肉眼可见的颈部淋巴结以及下颌骨进行病理学观察。对第５代移植瘤进行超微结构观察。第４代移植瘤及裸鼠骨髓细胞进行染色体检查。结果移植成功率为９０％，液氮冻存复苏移植成功率为１００％。移植瘤符合鳞状细胞癌特征，染色体检查表明其属人类染色体。４只裸鼠发生了口底和颌下浸润。１只转移至颌下淋巴结。癌细胞浸润肌肉，神经和周围血管。结论本模型较客观地反映了人舌鳞癌的生物学行为，为舌癌的生物学及实验治疗的研究提供了较理想的动物模型。
Objective To establish clinically relevant animal model of human tongue carcinoma.Methods Cell suspensions of human tongue squamous cell carcinoma cell line (Tca8113) were inoculated orthotopically into nude mice and the growing tumors were serially passaged by orthotopic transplantation. The oral cavity, mandible and neck of the nude mice were sectioned and examined histologically. The ultrastructure and chromosome of the tumors were also investigated. Results ①The success rate of transplant was 90%. ②The mouse growing tumors exhibited the same identical properties as the donor tumor. ③Human tongue cancer cells could grow in the homologous oral cavity site of nude mice, extended and metastasized to adjacent tissues and lymph node of the neck. Conclusion The model can be useful for evaluating biological behavior and new therapeutics for tongue cancer.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1998年第2期73-75,共3页
Chinese Journal of Stomatology
基金
湖北省自然科学基金
