摘要
目的筛选中子辐射后小鼠肠道损伤的差异基因。方法12只二级雄性BALB/c小鼠,3Gy中子照射后6h,取小鼠空肠组织,提取RNA,采用小鼠全基因组寡核苷酸芯片技术分析其基因表达谱的变化,mas系统对差异基因进行分类,RT-PCR验证芯片筛选的部分差异基因。结果中子照射后6h,在所分析的35 852条目的基因中,小鼠空肠组织中共有608个差异基因,其中上调的有277个,下调的有331个。mas系统的功能分类结果显示这些差异基因涉及到代谢、转运、消化、应激、细胞黏附、细胞生长发育和分化、细胞死亡、细胞运动、细胞通讯、细胞周期、DNA代谢和修复、生殖相关基因,另外还有一些未知的功能基因等。与中子辐射肠道损伤密切相关的有应激、细胞生长与死亡、信号转导、细胞周期、DNA损伤与修复等类别的基因。RT-PCR验证了部分结果,与芯片一致。结论中子辐射后,损伤的空肠组织中存在大量差异基因,表明中子辐射对肠道是一种多靶点的损伤;筛选出的差异基因可能为中子辐射肠道损伤的敏感基因和防治靶点。
Objective To screen the differentially expressed genes in the small intestine of mice after exposure to neutron irradiation. Methods Twelve male BALB/c mice were exposed to total body irradiation with fission neutron reactor for an absorption dosage of 3 Gy. Whole genome expression oligonucleotide microarray was performed 6 hours after irradiation to observe the changes in the whole genome in the murine small intestine. The differentially expressed genes were functionally classified by mas system. A part of the differentially expressed genes were further identified by RT-PCR. The integrating optical density of the electrophoretie bands was detected by the CMIAS image analysis system. Results Six hours after neutron irradiation, it was found that 608 of 35,852 genes observed by microarray differentially expressed in the small intestine, among them 277 genes were up-regulated and 331 genes were down-regulated. The differentially expressed genes could be functionally classified by mas system as metabolism, transport, digestion, stress, cell adhesion, cell growth, cell development and differentiation, cell death, cell motility, cell eomrnunieation, cell cycle, DNA metabolism and repair, reproduction and some unknown genes. Genes closely associated to intestinal injury induced by neutron irradiation were included in stress, cell growth and death, signaling, cell cycle, DNA repair and so on. RT-PCR partially confirmed the array results. Conclusion It is indicated that hundreds of genes have differentially expressed in the small intestine 6 hours after neutron irradiation, suggesting that multiple injury targets may exist in the intestine after being irradiated by neutron. Some of the screened out genes might serve as the target for the protection and therapy of intestinal injury induced by neutron irradiation.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2009年第1期98-101,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金资助项目(30700197
30870735)
全军"十一五"专项课题(06Z064)
关键词
空肠
中子
辐射损伤
实验性
寡核苷酸序列分析片
jejunum
neutron
radiation injuries, experimental
oligonucleotide array sequence analysis
