摘要
目的探讨狼疮肾炎(LN)不同本虚证候患者外周血单个核细胞(PBMCs)内肿瘤坏死因子-α(TNF-α)信号通路主要受体衔接蛋白-TNF相关死亡结构域蛋白(TRADD)、Fas相关死亡结构域蛋白(FADD)、受体相互作用蛋白-1(RIP-1)和TNF受体相关因子-2(TRAF-2)表达变化。方法确诊的51例LN住院患者辨证分型为阴虚内热证组(13例)、气阴两虚证组(26例)及脾肾阳虚证组(12例),设健康志愿者17名为正常组。收集外周静脉血,分离PBMCs,采用实时荧光定量PCR检测上述4种受体衔接蛋白、Caspase-3和白介素-1β(IL-1β)的mRNA表达水平。结果(1)与正常组比较,阴虚内热组、气阴两虚组、脾肾阳虚组TRADD mRNA表达均显著降低,分别为正常组的0.54、0.32和0.38倍(P<0.05,P<0.01),3组之间差异无统计学意义(P>0.05);(2)FADD mRNA表达分别为正常组的0.79、0.62和0.72倍,仅气阴两虚组降低差异具有统计学意义(P<0.05);(3)RIP-1 mRNA表达分别为正常组的0.79、0.50和0.60倍,气阴两虚组和脾肾阳虚组降低差异具有统计学意义(P<0.01,P<0.05),3组之间差异无统计学意义(P>0.05);(4)TRAF-2 mRNA表达分别为正常组的0.70、0.52和0.50倍,后两组降低差异有统计学意义(P<0.01),各LN组间差异无统计学意义;(5)3组LN患者PBMCs中Caspase-3 mRNA表达均显著升高(均P<0.01);(6)阴虚内热证组IL-1β mRNA显著降低,且显著低于气阴两虚及脾肾阳虚证组(均P<0.05)。结论LN各虚证患者PBMCs中TRADD、FADD、RIP-1和TRAF-2 mRNA表达均不同程度低于正常组,其中气阴两虚组、脾肾阳虚组降低幅度较阴虚内热证组相对较小。这些信号蛋白介导的凋亡信号可能参与了LN本虚证候的形成。
Objective To investigate the mRNA expressions of the TNF adapter proteins, including TNF receptor-associated death domain protein （TRADD）, Fas-associated death domain protein （FADD）, receptorinteracting protein 1 （ RIP-1 ） and TNF receptor-associated factor-2 （TRAF-2） in peripheral blood mononuclear cells （PBMCs） of lupus nephritis （LN） patients of various TCM asthenia syndromes. Methods Fifty-one inpa- tients with LN were differentiated according to TCM syndrome differentiation, 13 cases of yin-deficiency with inner heat syndrome （A） ; 26 cases of both qi-yin deficiency syndrome （B）, 12 cases of Pi-Shen yang-deficiency syndrome （C）. Peripheral venous blood samples from the 51 LN patients and 17 healthy subjects were collected to separate PBMCs. The mRNA expressions of TNF adapter molecules （TRADD, FADD, RIP-1 and TRAF-2）, as well as Caspase-3 and interleukin-1 β（IL-1 β） were analyzed by quantitative real-time PCR and the differences among them were compared. Results （1）As compared with the healthy subjects, expression of TRADD mRNA in patients of syndrome A, B and C was lowered to 0.54, 0.32, and 0.38-fold, respectively （ P 〈 0.05, P 〈 0.01）, showing insignificant difference among the three syndromes; （2） FADD mRNA lowered to0.79, 0.62, and 0.72-fold respectively, only with significance shown in syndrome B （ P 〈0.05） ; （3） RIP-1 mRNAIowered to 0.79, 0.50, and 0.60-fold respectively with significance shown in syndrome B and C （ P 〈0.01, P 〈0.05）, and insignificant difference was shown among the three syndromes; （4） TRAF-2 lowered to 0.70, 0.52, and 0.50-fold respectively （P〈0.01 ,P〈0.01 ,P=0.07）, significance shown in syndrome B and C （P〈0.01）, but with insignificant difference among the three; （5） Caspase-3 elevated in all patients of the three syndromes （all P 〈 0. 01 ） ; （6） IL-16 in syndrome A was apparently lower ed to the normal range and also lower than that in the other two syndromes （ both P
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2009年第1期34-39,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.30572290)
关键词
肿瘤坏死因子-Α
信号通路
受体衔接蛋白
狼疮肾炎
外周血单个核细胞
中医虚证
tumor necrosis factor-α
signal transduction
receptor adapter proteins
lupus nephritis
peripheral blood mononuclear cells
TCM asthenia syndrome
