摘要
亲和层析法分离纯化玉米精细胞质膜特异糖蛋白陈丽邵邻相汪洁杨中汉（北京大学生命科学学院，北京１００８７１）ＩｓｏｌａｔｉｏｎａｎｄＰｕｒｉｆｉｃａｔｉｏｎｏｆＳｐｅｒｍＣｅｌＰｌａｓｍａＭｅｍｂｒａｎｅＧｌｙｃｏｐｒｏｔｅｉｎｓｏｆＺｅａｍａｙｓｂｙＡ...
After hypo osmotic shock and percoll discontinuous density gradient centrifugation, mass active sperm cells were isolated from the fresh pollen grains of Zea mays .The crude plasma membrane proteins were extracted by the method of aqueous polymer two phase protion.In order to further purify the crude PM protein extracts,three different plant lectins(WGA,ConA and RCAⅠ)were coupled to the activated Sepharose 4B separately to three kinds of affinity gel columns(AC).The retained glycoproteins were eluted and enriched by ultrafiltration,and then isolated and identified by SDS PAGE.Through silver staining analysis,there were 7 protein constituents(62,58,37,34,22,18,15 kD)to bind specifically to WGA,6 constituents(68,66,60,39,37,34 kD)to ConA,and 1 constituent(67 kD)to RCA Ⅰ.The result indicated the existence of the glcNAc,mannosyl,and galactosyl residues in the plasma memberane glycoproeins of maize sperm cells.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第3期339-341,共3页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金
关键词
玉米
精细胞质膜
糖蛋白
亲和层析法
纯化
Zea mays L.Sperm cell,Plasma membrane glycoproteins,Affinity chromatography,SDS PAGE
