摘要
本实验应用斑点免疫金银染色（Ｄｏｔ－ＩＧＳＳ）及斑点酶联免疫吸附试验（Ｄｏｔ－ＥＬＩＳＡ）对５０例ＳＬＥ患者体内的肾小球基底膜抗体（ＧＢＭ－Ａｂ）及肾小管抗体（Ｔｕｂ－Ａｂ）进行了检测比较。结果表明：Ｄｏｔ－ＩＧＳＳ法检测ＧＢＭ－Ａｂ和Ｔｕｂ－Ａｂ的阳性率分别是６２％和７６％，Ｄｏｔ－ＥＬＩＳＡ法检测ＧＢＭ－Ａｂ和Ｔｕｂ－Ａｂ的阳性率分别是６８％和７８％。两法检查抗体阳性率间无明显差异。两法检查ＧＢＭ－Ａｂ的滴度无显著差异，而Ｄｏｔ－ＩＧＳＳ检查Ｔｕｂ－Ａｂ的滴度高于Ｄｏｔ－ＥＬＩＡＳ检查，两法有显著性差异（ｐ＜０．０５）。以上结果提示：Ｄｏｔ－ＩＧＳＳ法或Ｄｏｔ－ＥＬＩＳＡ法检测ＧＢＭ－Ａｂ和Ｔｕｂ－Ａｂ虽有相似的敏感性，但从测定抗体滴度等方面考虑，Ｄｏｔ－ＩＧＳＳ法优于Ｄｏｔ－ＥＬＩＳＡ法。
In the experiment, glomerular basement membrane antibody (GBMAb) and renal tubular antibody (TubAb) of 50 patients with SLE were detected by dotimmunogold silver staining (DotIGSS) or dotenzyme linked immunosorbent assay (DotELISA). The result showed that the positive rates of detecting GBMAb and TubAb by DotIGSS or by DotELISA were 62% and 76% or 68% and 78% respectively.The difference between positive rates of measuring GBMAb and TubAb in the two methods were insignificance. Moreover,the detecting antibody titers by DotIGSS were higher than those by DotELISA. The difference of GBMAb titers between the two methods was insignificance,but the difference of TubAb between the two methods was significance (P<0.05).The results indicate that although detecting GBMAb and TubAb by DotIGSS or DotELISA have similar sensitivity, the method of DotIGSS is better than that of DotELISA on measuring the titers of GBMAb and TubAb.
出处
《基础医学与临床》
CSCD
1998年第1期27-30,共4页
Basic and Clinical Medicine
