摘要
在pH 7.2 Na2HPO4-NaH2PO4缓冲溶液及聚乙二醇-6000存在下,氰戊菊酯（Fen）与兔抗氰戊菊酯抗体（Fen-Ab）发生免疫反应,形成具有一定疏水性的免疫复合物微粒,在350,390,420,440,480 nm产生5个共振散射峰,其中390 nm处的共振散射峰最强。研究了pH、聚乙二醇-6000、Fen-Ab浓度、免疫反应温度和时间对测定Fen的影响。在最佳条件下,氰戊菊酯（Fen）浓度c在0.20-6.40μg·mL^-1范围内与390 nm处的散射强度ΔIRS呈线性,回归方程、相关系数、检测限分别为ΔIRS=23.05c-1.39,0.997 8,0.07μg·mL^-1Fen。干扰试验结果表明,该法具有较好的选择性。该法用于废水中Fen分析,回收率在92.91%-101.25%之间,相对标准偏差在1.71%-4.80%之间。
In the pH 7.2 Na2HPO4-NaH2PO4 buffer solutions and in the presence of PEG-6000,fenvalerate（Fen） antisera was combined with Fen specifically,and aggregated to form immune complex particles that exhibited five resonance scattering peaks at 350,390,420,440 and 480 nm respectively.The peak at 390 nm was the strongest and was chosen for use.Fen concentration（c） in the range of 0.20 to 6.40 μg·mL^-1 was proportional to the resonance scattering intensity at 390 nm.Its regression equation was ΔIRS=23.05c-1.39,the correlation coefficient was 0.997 8,and the detection limit was 0.07 μg·mL^-1.Effects of buffer solution type,pH value,buffer solution volume,fenvalerate antisera concentration,PEG-6000 concentration,incubation temperature and time on the resonance scattering intensity were considered in detail.With pH（5.8-8.0）increasing,the IRS and Ib all decreased.When the pH value was at 7.2,the ΔIRS was bigger.Three buffer solutions of pH 7.2,including Na2HPO4-citric acid,Na2HPO4-KH2PO4 and Na2HPO4-NaH2PO4,were examined.The pH 7.2 Na2HPO4-NaH2PO4 buffer solution gives the biggest ΔIRS value.PEG-6000 could enhance the ΔIRS value.When the concentration of PEG-6000 was 50.0 mg·mL^-1,the ΔIRS was achieved at max.Fen was a stable chemical.The IRS increased within 20 min,while the ΔIRS remained constant when incubation time was in the range of 20-40 min.The condition of a pH 7.2 Na2HPO4-NaH2PO4 buffer solution-50.0 mg·mL^-1 PEG-60006.67 μg·mL^-1 Fen antisera-30 ℃-incubation time 20 min was chosen.According to the procedure,the influence of foreign substances on the determination of 1.60 μg·mL-1 Fen was examined,within a relative error of ±5%. Results showed that the following coexistent substances had no impact on the RS assay： 96 μg·mL^-1 ametryne,96 μg·mL^-1 m-aminotoluene,48 μg·mL^-1 simetryne,48 μg·mL-1 p-aminotoluene,80 μg·mL^-1 BSA,80 μg·mL^-1 HSA,80 μg·mL^-1 Fe^3＋,80 μg·mL^-1 Mg^2＋,160 μg·mL^-1 Ca^2＋,and 160 μg·mL^-1 glucose.The results indicated that this RSS assay
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2009年第1期214-216,共3页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(20667001)
广西新世纪十百千人才基金项目资助
关键词
氰戊菊酯
免疫反应
共振散射光谱
Fenvalerate
Immune reaction
Resonance scattering spectral method
