摘要
以2,4-二氯酚(2,4-DCP)和2,4,6-三氯酚(2,4,6-TCP)为代表,采用Sol-gel法固定化漆酶处理氯酚类污染物.结果表明:底物初始浓度为0.5 mmol/L,固定化漆酶投加量为7 g/L,反应3 h后DCP去除率可达95.3%,而反应2 h后TCP去除率可达99.6%.TCP和DCP去除过程可以用米氏方程描述,其米氏常数Km值分别为11.72和9.97 mmol/L,因此固定化漆酶对TCP的亲和力高于DCP,这导致了在反应时间、初始浓度、固定化酶投加量以及底物共存等因素变化后两者去除效果上的差异.连续48 h的运行数据和酶活损失表明固定化漆酶具有较好的反应稳定性.
2, 4 - DCP and 2, 4, 6 - TCP chosen as model of chlorophenols, the treatment process of them with immobilized laccase by Sol- gel showed good removal effect and response stability. On the condition of the initial substrate concentration of 0.5 mmol/L and immobilized laccase dosage to 7g/L, DCP removal rate up to 95.3% after 3 hours and TCP removal rate up to 99.6% reaction after 2h, showed good treatment effect. The degradation procession of them could be described with Michaelis - Menton Formulation with Km value of DCP and TCP being 11.72 and 9.97 mmol/L respectively. The affinity of TCP with immobilization of Laccase was higher than its of DCP, which leaded to different of removal effect under the variable conditions, i. e. , reaction time, initial concentration, dosage of immobilized laccase and mixture. Data of continuous operation within 48 hours and the loss activity of immobilized laccase showed its good reaction stability.
出处
《福州大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第6期910-914,共5页
Journal of Fuzhou University(Natural Science Edition)
基金
福建省重大科技计划资助项目(2006SZ010080044)
