摘要
目的研究正常胎鼠胚胎发育13～16d泄殖腔凋亡细胞的动态分布情况，了解细胞凋亡在泄殖腔胚胎发育过程中的作用。方法利用TUNEL染色技术检测wistar大鼠胚胎13～16d泄殖腔凋亡细胞的分布情况。结果正常胎鼠胚胎13d尿直肠隔上皮层和间质区域可见凋亡细胞。随着胚胎发育，尿直肠隔间质的凋亡细胞逐渐增多并向下延伸，腹侧间质比背侧间质凋亡细胞明显；直肠背侧间质可见大量凋亡细胞。胚胎14d，直肠末端和未来肛门开口处的泄殖腔膜开始出现凋亡细胞。胚胎15d，尿直肠隔与泄殖腔膜融合，尿直肠隔间质内的凋亡细胞一直向下延伸到融合部位。胚胎16d，肛膜裂解，直肠与外界相通。结论在泄殖腔的胚胎发育过程中，尿直肠隔的下降、融合过程、泄殖腔的构型变化和肛膜的裂解，细胞凋亡均发挥重要作用。
Objective To investigate the role apoptosis in hindgut morphogenesis in normal E13 to E16 rat embryos. Methods TUNEL staining was performed to detect the apoptotic cells in the cloaca of wild-type E13 to E16 rat embryos. Results On E 13,the genital tubercle has fully developed and the cloaca is well formed. The urogenital septum （URS） divided cloaca into urogenital sinus and rectum. The tail groove were formed. There were apoptotic cells in the epithelial layers and mesenchyme region of URS. Following the development, genital tubercle grew gradually and shifted ventrocaudally. The tail groove deepened gradually and cloacal membrane rotated dorsalcaudally. The apoptotie cells increased and they descended in the mesenchyme region of URS. Many apoptotic cells appeared in the dorsal mesenchyme of the rectum. On E14,apoptotic cells began to appear at the terminal rectum and the future anal orifice part of cloacal membrane. On E15,URS fused with cloacal membrane and apop tosis in URS descended to the fusion position. Anal membrane ruptured and rectum communicated with the exterior on E16. Conclusions In cloacal embryological developments, apoptosis is involved in the descend and fusion of URS, the configuration transformation of cloaca, and the rupture of cloacal membrane.
出处
《中华小儿外科杂志》
CSCD
北大核心
2009年第1期35-38,共4页
Chinese Journal of Pediatric Surgery
基金
基金项目：国家自然科学基金资助（批准号：30400473、30872704）
