摘要
目的:研究天津地区呼吸道分离肠杆菌科细菌中产质粒介导超广谱β-内酰胺酶(ESBLs)菌株基因分布情况。方法:收集天津市胸科医院2004年临床分离产ESBLs肠杆菌科细菌,选取携带质粒菌株,提取其质粒DNA作为模板,采用聚合酶链反应(PCR)和序列分析确定ESBLs的基因型。结果:40株接合试验阳性产ESBLs菌株中TEM型、SHV型、CTX-M型阳性率分别为50.0%、20.0%和67.5%,PCR阳性菌株中同时产2种以上酶者高达61.3%。随机抽取5株ESBLs的PCR产物进行序列分析,其中2株TEM型的序列均属TEM-1型,1株SHV型的序列属于SHV-12型,2株CTX-M型的序列分属CTX-M3、CTX-M14型。结论:天津地区呼吸道肠杆菌科细菌ESBLs基因分型以CTX-M型为主,同时产2种以上酶的菌株居多数。
Objective： To study the genotype of the clinical isolates of Enterobacteriaceae producing plasmid-mediated extended-spectrum β-lactamases （ESBLs） from respiratory tract infection in Tianjin. Methods： PCR and DNA sequencing were used to detect the genotype of ESBLs in Tianjin Chest Hospital in 2004. Results： PCR experiment showen 50.0%blaTEM, 20.0% blaSHV, 67.5%blaCTX-M-positive results for 40 clinical strains producing ESBLs. Among strains producing positive PCR results, those who produced β-1actamases with more than 2 genotypes was up to 61.3%. Five PCR products were identified as TEM-1,TEM-1,SHV-12,CTX-M3 and CTX-M14. Conclusion： CTX-M type is the predominant genotype of ESBLs in clinical Enterobacteriaceae strains from respiratory tract infections in Tianjin, and most of them produced β-lactamases with more than 2 genotypes.
出处
《天津医药》
CAS
北大核心
2009年第1期17-20,共4页
Tianjin Medical Journal
关键词
肠杆菌科
呼吸道感染
质粒
Β内酰胺酶类
基因型
天津
enterobacteriaceae respiratory tract infections plasmids beta-lactamases genotype TIANJIN
