摘要
目的检测肿瘤干细胞标志CD166在大肠癌中的表达，以了解其在大肠癌发病中的意义。方法用RT—PCR检测23例大肠癌患者的癌组织和癌外正常大肠组织中的CD166 mRNA的阳性率和表达水平，并分析其与临床和病理各变量的相关性。结果大肠癌组织CD166 mRNA表达的阳性率为95．7％，表达水平为0．309±0．145，均显著高于正常大肠组织的39．1％和0．109±0．255（P〈0．01），其表达率与表达强度与患者的年龄、性别、临床分期、肿瘤部位、肿瘤大小、病理分型等均无显著关系（P〉0．05）。结论肿瘤干细胞标志物CD166在大肠癌中高表达，其表达与临床和病理的特征无显著关系。
Objective To evaluate the expression of CD166 in colorectal carcinoma （CRC） tissues in order to explore its significance in CRC pathogenesis. Methods The expression of CD166 mRNA was detected by RT-PCR in CRC tissues and normal coloreetal tissues outside the cancers in 23 patients with confirmed CRC. Its association with clinicopathologieal parameters was analyzed. Results The positive rate （95.7%） and expression level （0. 309 ±0. 145 ） of CD166 mRNA in CRC tissues were statistically higher than those of 39. 1% and 0. 109 ±0. 255 in the normal colorectal tissues （P 〈 0.01 ）. No significant association of CD166 mRNA expression with age, gender, Dukes stages, tumor locations, tumor sizes or histological types of CRC was found. Conclusion CD166 mRNA expression is up-regulated in CRC tissues, which is not associated with clinicopathological parameters.
出处
《中华生物医学工程杂志》
CAS
2008年第4期278-280,共3页
Chinese Journal of Biomedical Engineering
基金
广东省科技基金面上项目（2004-139-50）
广州市医药卫生科技项目（2007-YB-004）
