摘要
目的为探讨甲醛致生物机体DNA-蛋白质交联(DPC)作用及其修复能力。方法以3月龄SPF级健康成年雄性Wistar大鼠为实验动物分别进行体内(0、0.5、1.0、3.0mg/m3气态甲醛吸入染毒72h)和体外实验(0、25、50、100、150、200μmol/L液态甲醛染毒1h),并进行体内(3.0mg/m3的气态甲醛吸入染毒72h)和体外(75μmol/L的液态甲醛染毒1h)修复实验(修复0、6、12、18和24h)。采用KCl-SDS沉淀法检测大鼠肺、肾组织细胞DPC率。结果体内实验表明,低浓度的气态甲醛(0.5mg/m3)不能明显诱导大鼠肺、肾组织细胞产生DPC,较高浓度的气态甲醛(≥1.0mg/m3)可以产生明显的DPC作用(P<0.05);由3.0mg/m3浓度的气态甲醛使肺、肾组织细胞产生的DPC可在24和18h内得到修复,并可在24h内恢复到空白对照水平。体外实验表明,低浓度的液态甲醛(25μmol/L)不能明显诱导大鼠肺、肾组织细胞产生DPC,较高浓度的液态甲醛(≥50μmol/L)可以产生明显的DPC作用(P<0.05);由75μmol/L浓度的液态甲醛使肺、肾组织细胞产生的DPC可以在18和12h内得到修复,24h内可以恢复至空白对照水平。结论较低浓度的甲醛不能明显诱导DPC的形成,而较高浓度的甲醛则能显著诱导DPC的形成,甲醛所致的DNA-蛋白质交联可得到修复,且体外修复比体内修复时间要短。
Objective To explore the DNA-protein crosslink （DPC） induced by formaldehyde （FA） and the repair. Methods Three-month-old Wistar male rats were used as the experimental animals and exposed to gaseous FA at the doses of 0, 0.5, 1.0, 3.0 mg/m^3 respectively through continuous inhalation for 72 h. The cellular suspension of the lungs and kidneys was exposed to FA at the doses of 0, 25, 50, 100, 150 and 200 umol/L respectively for 1 h. The KCl-SDS assay was used to detect the coefficient of DPC in the lung and renal cells. The repair at 0, 6, 12, 18 and 24 h exposure points was determined in the exposure test of 3.0 mg/m^3 for 72 h in vivo and 75 umol/L for 1 h in vitro. Results Higher gaseous FA （≥ 1.0 mg/m^3） could significantly cause DPC （P〈0.05）. DPC induced by gaseous FA of 3.0 mg/m^3 could be repaired within 24 and 18 h in the pulmonary and renal cells respectively （P〈 0.05） and be restored to the level of control group around 24 h. Higher concentration of liquid FA（≥50 umol/L） could significantly cause DPC （P〈0.05）. DPC induced by liquid FA of 75 umol/L could be repaired in 18 h（P〈0.05） and be restored to the level of the control group around 24 h. Conclusion Higher FA exposure can induce DNA-protein crosslink in vivo （in vitro） and it can be repaired, the DPC-repaired time in vitro is shorter than that in vivo.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2009年第1期37-39,42,F0003,共5页
Journal of Environment and Health
基金
国家科技支撑计划项目(2006BAI19B05-2)
