摘要
目的探讨大鼠颈动脉重度狭窄合并脑梗死后脑组织促红细胞生成素(EPO)的变化。方法用针控线拴法和自体血栓制作大鼠颈动脉狭窄合并多发脑梗死模型,免疫组织化学染色定位及半定量检测EPO的变化。结果颈动脉狭窄合并脑梗死无再通组梗死侧脑组织在第3天时EPO表达逐渐增高,与非梗死侧相比差异有统计学意义;第5天时达高峰,第7天时下降到最低点,明显低于非梗死侧,14d时EPO再次增高,但与非梗死侧相比差异无统计学意义。颈动脉狭窄合并脑梗死1d再通后24h梗死侧EPO有相对高的表达,2d和3d再通后24h梗死侧EPO与非梗死侧相比无明显增高,第5天达高峰,7d时开始下降与非梗死侧相比差异无统计学意义。颈动脉狭窄合并脑梗死再通组与无再通组梗死侧EPO表达的比较只有在第7天时再通组明显高于无再通组。结论颈动脉重度狭窄合并脑梗死再通时会刺激一些内源性保护物质的生成,且此内源性保护物质的生成也与再通的时间有关。
Objective To explore the expression of erythropoietin （EPO） in rats following carotid artery severe stenosis complicated with multiple cerebral embolism. Methods In rats, severe stenosis of the common carotid artery （CCA） was induced by ligation of common carotid artery using a needle to control the degree of stenosis, and multiple cerebral emboli were induced by injecting autogenic embolus through the external carotid artery. The expression of EPO was then determined by immunohistochemistry assay. Results In non-recanalized CCA stenosis, level of EPO expression in affected hemisphere became significantly higher （P〈0.05） than that in non-ischemic hemisphere starting from day 3, peaked on day 5, declined on day 7 to a nadir that was obviously below the level of contralateral side, and then increased again on day 14 but did not produced a statistical difference between the both hemispheres. In recanalized CCA stenosis, the expression of EPO in ischemia hemisphere were significantly higher （P〈0.05） compared with non-ischemic hemisphere at the 1st day after CCA stenosis, peaked at the 5th day, and then decreased at 7th day. Compared with CCA stenosis group, the expression of EPO increased markedly in recanalization group at 7th day （P〈 0.05）. Conclusion Endogenous neuroprotection may be induced after carotid artery severe stenosis complicated with multiple cerebral embolism and recanalization. The neuroprotective effects may be related to the time of recanalization.
出处
《中国药物与临床》
CAS
2009年第1期5-8,81,共5页
Chinese Remedies & Clinics
基金
国家自然科学基金(30670725)
