摘要
目的检测胰岛素样生长因子Ⅱ(IGF-Ⅱ)与基质金属蛋白酶9(MMP-9)在血清及胎盘中的表达,探讨二者的关系及其在子痫前期发病中的作用。方法(1)用酶联免疫吸附法(ELISA)检测30例子痫前期患者(子痫前期组,其中轻度子痫前期20例、重度子痫前期10例)及15例正常晚期妊娠孕妇(对照组)的血清中IGF-Ⅱ及MMP-9的水平。(2)用免疫组化方法检测两组孕妇胎盘组织中IGF-Ⅱ及MMP-9的表达,用计算机图像分析系统进行定量分析比较。结果(1)子痫前期组血清IGF-Ⅱ及MMP-9水平均明显低于对照组(P<0.01,P<0.01)。(2)IGF-Ⅱ及MMP-9在子痫前期组胎盘中的表达量均显著低于对照组(P<0.01,P<0.01)。(3)子痫前期组患者血清及胎盘组织中IGF-Ⅱ水平分别与相应部位的MMP-9水平呈正相关(r=0.379,P<0.05;r=0.442,P<0.05)。结论孕妇血清及胎盘组织中IGF-Ⅱ、MMP-9水平变化与子痫前期发病及病情发展有关。
Objective ： To investigate the expression of insulin like growth factor - Ⅱ （ IGF - Ⅱ ） and matrix - metalloproteinase - 9 （ MMP - 9 ） in human serum and placenta and to evaluate their role in the pathogenesis of preeelampsia. Methods ： （ 1 ） The levels of IGF - Ⅱ and MMP -9 in serum were detected from 30 patients with preeelampsia （preeclampsia group） and 15 normal pregnant women （control group） by enzyme linked immunosorbent assay （ELISA）. （2） The eelluar distribution and quantity of IGF - Ⅱ and MMP -9 in normal and preeelampsia placenta was determined by immunohistochemistry. The mean density was determined by image analysis technique. Results ： （ 1 ） Serum levels of IGF - Ⅱ and MMP - 9 in preeclampsia group were both significantly lower than that in control group （ P 〈0. 01, P 〈0. 01 ）. （2） The IGF- Ⅱ and MMP-9 mean densities of preeclampsia placenta were both significantly lower than that of normotensives （ P 〈0. 01, P 〈0. 01 ）. （3） The levels of IGF- Ⅱ in serum and placenta had a significant positive relationship with that of MMP - 9 in preeclampsia group （ r =0. 379, P 〈 0. 05 ; r = 0. 442, P 〈 0. 05 ）. Conclusion ： The changes of levels of IGF- Ⅱand MMP-9 are related to the occurrence and development of preeclampsia.
出处
《中国优生与遗传杂志》
2009年第1期24-26,共3页
Chinese Journal of Birth Health & Heredity
