摘要
目的:构建大鼠酒精性肝损伤动物模型,观察葛根提取物对大鼠酒精性肝损伤的治疗作用。方法:健康SD大鼠48只,雌雄各半,体重180～220g。随机分为四组,每组12只。对照组和模型组分别用蒸馏水(10ml/kg·d-1)和56%酒精(10ml/kg·d-1)灌胃,连续12周;治疗组和治疗对照组给予56%酒精(10ml/kg·d-1)灌胃,连续12周,治疗组在第9周开始用葛根提取物灌胃(10ml/kg·d-1),治疗对照组用等体积的生理盐水灌胃。在12周末全部处死大鼠,检测大鼠血清ALT、AST及铁含量,检测肝组织和血清SOD活力及MDA含量。结果:模型组大鼠较对照组大鼠精神状态差、毛发光泽差、食欲差、大便溏泻、反应迟钝、而且活动减少,血清ALT、AST及铁含量高于对照组(P<0.01),肝组织SOD活力低于对照组(P<0.01)、MDA含量高于对照组(P<0.05),治疗组大鼠较治疗对照组精神状态好,血清ALT、AST及铁含量低于对照组(P<0.01),血清及肝组织SOD活力高于治疗对照组、MDA含量低于治疗对照组(P<0.05)。结论:葛根具有一定对抗酒精所致的肝损伤作用。
Objective： To construct the animal model of alcoholic liver damage and observe the therapeutic effects of Radix Puerariae on the alcoholic liver injury in rats. Methods： 48 SD rats, half males and half females, 180-220g of weight, were randomly divided into 4 groups, 12 for each group. Rats of the experiment group were given saline infusion [ 10ml/（kg·d） 3and 56% ethanol infusion [ 10ml/（kg.d）]was given to the control group for 12 weeks. From the 9m week, the experiment group was given Pueraria in gastric, and the control group were given the same quantity of physiological saline. By the end of the 12^th week, all rats were sacrificed and their serum was detected for ALT, AST and iron. Superoxide dismutase （SOD） activity and malonaldehyde （MDA） content were determined in hepatic tissue and serum. Results： Contrasted with the control group, rats in the experiment group had worse mental state, dim coat, poor appetite, diarrhea, clumsy reaction, and the times of movement reduced. ALT, AST and iron in rats serum were higher than that in control group （P〈0.01）. Compared with the control group, SOD activity was significantly decreased in model group （P〈0.01）, and MDA level was significantly increased in model group （P〈0.05） in hepatic tissue and serum. Compared with the treatment control group, ALT, AST and iron in rats serum were lower in Pueraria treated group （P〈0.01）, SOD activity was significantly increased in model group （P〈0.05）, MDA level was significantly decreased in model group （P〈0.05） in hepatic tissue and serum. Conclusion： Pueraria had therapeutic effects on the alcoholic liver injury in rats.
出处
《大理学院学报（综合版）》
CAS
2008年第12期28-30,33,共4页
Journal of Dali University
关键词
酒精性肝损伤
葛根
治疗
alcoholic liver damage
Pueraria
treatment
