摘要
目的:探讨姜黄素对糖尿病大鼠肾脏病变的作用及对肾脏血红素加氧酶-1(HO-1)表达的影响。方法:诱导大鼠糖尿病后,随机分为对照组(n=10)及治疗组(n=10),比较各组的血生化、尿微量白蛋白排泄量。采用逆转录多聚酶链反应(RT-PCR)检测HO-1 mRNA。用免疫组化检测HO-1蛋白的定位和表达水平。结果:糖尿病大鼠内生肌酐清除率(Ccr)、尿微量白蛋白(mAlb)排泄量较正常鼠增多,HO-1 mRNA和蛋白表达无明显上调。HO-1蛋白主要分布于肾小管、髓质的集合管及髓袢的上皮细胞中,肾小球表达极少。姜黄素治疗能明显降低Ccr,减少mAlb排泄量,诱导HO-1 mRNA和蛋白的表达。结论:姜黄素对糖尿病大鼠肾脏病变具有明显的防治作用,其机制可能是通过诱导HO-1的表达。
Objective： To explore the effects of Curcumin on renal lesions and its influence to expression of heme oxygenase-1 （HO-1） in the kidneys of diabetic rats. Methods： Diabetic rats were randomly divided into control （n= 10） and experimental group （n= 10） （treated with Curcumin）, blood biochemical parameters and 24 h urinary albumin excretion were compared. The level of HO-1 mRNA was examined by RT-PCR. The site and level of expression of HO-1 protein were examined by immunohistochemistry staining. Results： Compared with that in normal rats respectively, Ccr and urinary albumin excretion increased markedly in diabetic rats, but the expression of HO-1 mRNA and protein were not increased. HO-1 protein was mainly expressed in renal tu. bular cells, collecting ducts in medulla and Henle＇s loop. Ccr and urinary albumin excretion were decreased markedly and the expression of mRNA and protein of HO-1 were induced by the treatment of Curcumin. Conclusion： Curcumin has therapeutic effects on diabetic nephropathy. The mechanism is, at least in part, through induction of HO-1.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2009年第1期43-46,F0003,共5页
Medical Journal of Wuhan University
