摘要
目的观察大黄蟅虫丸对胰岛素样生长因子(IGF-1)在动脉粥样硬化(AS)模型大鼠中表达的影响,并从中探讨大黄蟅虫丸抗AS的机制。方法SD雄性大鼠体重220～300g,随机分为4组,对照组、模型组、大黄蟅虫丸组高剂量、低剂量组,每组8只,共32只。用高脂饲料喂养并建立大鼠AS模型,用大黄蟅虫丸灌胃12w后,观察各组大鼠血清血脂(TG,TC,HDL-C,LDL-C),丙二醛(MDA),超氧化物歧化酶(SOD),IGF-1含量的变化。结果模型组IGF-1含量显著降低,与对照组比较有显著差异(P<0.05);大黄蟅虫丸组较模型组血清IGF-1含量明显升高(P<0.05)。结论大黄蟅虫丸通过提高血清IGF-1表达,抑制血管平滑肌细胞的凋亡,发挥抗AS的作用。
Objective To observe the level of insulin-like growth factor （IGF）-1 in atherosclerosis （AS） model mice and investigate the anti-AS mechanism of Dahuang Zhechong pill. Methods The mice were divided into control group, model group and low and high dose Dahuang Zhechong treated group. AS model was made by high fat feeding and low and high dose Dahuang Zhechong treated group were given 0. 7 and 1.4 g/kg Dahuang zhechong pill respectively. After 12 w, the level of serum total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （ HDL-C）, low density lipoprotein cholesterol （ LDL-C ）, superoxide dismutase （ SOD）, malonaldehyde （MDA）, and IGF-1 were observed. Results Compared with model group, the level of TC, TG, LDL-C in control group were lower and HDL-C was higher remarkably （P 〈0. 01, P 〈0. 05）, while the level of TG and LDL-C in low dose Dabuang Zhechong treated group were lower （P 〈0. 01, P 〈0. 05） and the level of TG, TC, LDL-C in high dose Dahuang Zhechong treated group were lower than those of model group and HDL-C was higher than that of model group （P 〈 0. 01, P 〈 0. 05）. The activity of SOD in model group was lower than that of control and Dahuang Zhechong treated group （P 〈 0. 01, P 〈 0. 05 ）, and the content of MDA in model group was higher than that of control and high dose Dahuang Zhechong treated group （P 〈 0. 01 ）. The level of IGF-1 in model group was remarkably decreased than that of control and high dose Dahuang Zhechong treated group （P 〈 0. 05）. Conclusions Dahuang Zhechong pill could have the effect of anti-AS by increasing the level of IGF-1 and inhibiting the apoptosis of the vascular smooth muscle cell.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第1期39-41,共3页
Chinese Journal of Gerontology
基金
河北省教育厅资助(No.20042011)
河北省中医管理局(No.05163)
