摘要
目的建立一种接近临床特点的运动性哮喘动物模型。方法豚鼠27只分4组。实验组(A组)用脂多糖(1mg/kg)和甲双吡丙酮(50mg/kg)腹腔注射,4天后测定气道阻力和动态肺顺应性基础值,8小时后进行运动试验并复测上述指标。对照组有3组(B、C、D组);分别为腹腔注射脂多糖和甲双吡丙酮不运动组、腹腔注射生理盐水运动组和腹腔注射生理盐水不运动组。结果实验组豚鼠在运动后肺阻力增高、动态肺顺应性降低,而3个对照组上述指标变化均无统计学意义。
Objective To investigate the relationship between airway inflammation and exercise induced asthma (EIA), an experimental model for EIA in guinea pigs was developed. Method 27 guinea pigs was devided into 4 groups. A)LPS+MET exercise group ( n =7) had been pretreated with lipopolysaccharide (LPS 1 mg/kg, ip) and metyrapone (MET 50 mg/kg,ip) on the first day. Then lung resistance (RL) and dynamic compliance (Cdyn) were measured before and after exercise on the fourth day. B)LPS+MET non exercise group ( n =6) that pretreated with LPS and MET had nonexercise on the 4th day. C)NS exercise group ( n =7) pretreated with normal saline (NS 1 mg/kg, ip) on the 1st day. RL and Cdyn were measured before and after exercise on the 4th day. D)NS nonexercise group ( n =7) that pretreated with NS had no exercise on the 4th day.Result In LPS+MET exercise group RL increased and Cdyn reduced significantly after exercise. In another 3 control groups there were no such changes either in RL or in Cdyn. Conclusion These observations suggest that the treatment with LPS and MET could make a model for exercise induced asthma.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1998年第3期144-146,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
哮喘
脂多糖
甲双吡丙酮
运动性哮喘模型
Asthma Lipopolysaccharide Metyrapone Guinea Pigs Exercise induced Asthma