摘要
【目的】建立适合葡萄悬浮细胞系快速稳定生长的培养体系。【方法】以鲜食葡萄巨峰的果皮为材料，以B5为基本培养基，通过选择合适的激素配比及浓度、pH、蔗糖浓度等建立并优化葡萄愈伤组织培养体系及细胞悬浮培养体系。【结果】在B5＋0．5mg／L 6-BA＋NAA条件下，NAA最适质量浓度为1mg／L；在B5＋0.5mg／L6-BA＋2，4-D条件下，2，4-D最适质量浓度为1．O～2．0mg／L；在B5＋1mg／L NAA＋6-BA条件下，6-BA最适质量浓度为0．5～1．0mg／L；在B5＋2mg／L2，4-D＋KT条件下，KT的最适质量浓度0.2mg／L。在最佳蔗糖质量浓度30g／L、最适pH5．5～5．8培养基条件下，建立了能快速生长的均一、稳定的巨峰葡萄细胞悬浮体系，优化了培养条件。【结论】适合葡萄愈伤组织生长的培养体系为：B5＋250mg／L水解酪蛋白＋0．1mg／L NAA＋0．2mg／L KT＋30g／L蔗糖＋6g／L琼脂；适合葡萄细胞悬浮培养的体系为：B5＋1mg／L NAA＋0．5mg／L6-BA＋30g／L蔗糖和B5＋250mg／L水解酪蛋白＋0．1mg／L NAA＋0．2mg／L KT＋30g／L蔗糖。
[Objective] The researth establishd a suitable method for the stable and rapid growth of grape suspension culture. [Method] With the fruits of Grape Kyoho （V. vinifera L. ） as materials,B5 medium as basic medium, at the condition of proper hormone concentration and ratio, pH and sucrose to induce callus,a suspension culture system was established and optimized. [Result] The hormone concentration was determined in such a way：NAA 1 mg/L,at the condition of BS＋0.5 mg/L 6-BA＋NAA;2,4-D 1.0-2.0 mg/L,at the condition of B5＋0.5 mg/L 6-BA ＋2,4-D;6-BA 0.5-1.0 mg/L,at the condition of B5＋1 mg/L NAA＋BA;KT 0.2 mg/L,at the condition of B5＋2 mg/L 2,4-D＋KT. Their effects compared the proper hormone ratio was decided, and the rapid growth, equal, and stabilized grape suspension culture was established at the condition of pH 5.8 and sucrose 30g/L. [Conclusion] The instance of callus induction and suspension celll system establishment of Grape Kyoho indicate, the suitable medium for inducing callus is B5＋250 mg/L hydrolyzed casein＋0.1 mg/L NAA ＋0. 2 mg/L KT＋30 g/L sucrose＋6g/L agar,while suitable liquid medium for suspension culture for Kyoho is B5＋0.5 mg/L 6-BA＋1 mg/L NAA＋30 g/L sucrose and B5＋250 mg/L hydrolyzed casein ＋0.1 mg/L NAA ＋0.2 mg/L KT＋30 g/L sucrose.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第1期199-204,210,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
陕西省"13115"科技创新工程重大科技专项计划"葡萄与葡萄酒产业关键技术研究"(2007ZDKG-09)
