摘要
以甲醇作溶剂，提取料液比为1：10，超声提取2次（每次30rain）；以香草醛冰醋酸-高氯酸为显色剂，（显色温度70℃，显色时间15min）在541nm下，用紫外分光光度法测定总皂苷的含量。结果表明，无患子总皂苷含量在28～126μg之间与吸光度线性关系良好，相关系数r=0．9997，方法平均回收率为98．68％，RSD（n=5）为2．06％。本方法重现性好，结果稳定可靠，可为无患子果皮中总皂苷含量的测定提供参考。
The total saponins in pericarps of Sapindus mukorossi Gaertn were extracted twice with methanol for ultrasonic （ every 30 min a time） ,at a solid-liquid ratio of 1： 10. Then reacted with vanillin acetic acid-perchloric acid at 70 ℃ in 30 min, and the wavelength of UV is 541 nm. The results showed that it has a good linear relationship with absorbance （r =0.997） when the content of total saponins were at the range of 28-126 μg. The average recovery was 98.68% and 2.06% （ n = 5 ）. This method is reliable, accurate and steady with a good repeatability, and provided a reference for determination of total saponins in pericarps of Sapindus mukorossi Gaertn.
出处
《天然产物研究与开发》
CAS
CSCD
2008年第B05期84-86,共3页
Natural Product Research and Development
关键词
无患子
总皂苷
超声提取
齐墩果酸
紫外分光光度法
Sapindus mukorossi Gaertn
total saponins
ultrasonication extraction
oleanolic acid
UV spectrophotometry
