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疏水性石油烃降解菌的PCR测序及营养平衡研究 被引量:2

PCR Sequencing and Nutrient Balance of Hydrophobic Hydrocarbon Degrading Bacteria
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摘要 细胞表面疏水性是在研究细胞黏附物体表面领域最值得研究的细胞表面特性,研究表明其对疏水性有机物的去除率呈相关性。文章在对疏水性石油烃降解菌HDB-1生理生化特征、疏水能力及降解能力研究的基础上,继续对其16SrDNA序列进行分析,同时对其的营养平衡进行讨论。实验表明,PCR测序与细胞分类法得到的结果一致,菌株HDB-1属于假单胞菌属细菌(Pseudomonas),氮、磷则是影响HDB-1菌降解石油的限制因素。 Cell-surface hydrophobicity as one of surface properties of the most worthy studied was related definitely with degradation of organic pollutant. Based on the study of physiological-biochemical characters as well as hydrophobic capability and degradation capability, the sequence of 16SrDNA and nutrient balance of hydrophobic hydrocarbon degrading bacteria named HDB-1 were analyzed, experimental results showed that PCR sequencing was consistent with cell classification, and hydrophobicities of HDB-1 belong to Pseudomonas. Concentration of nitrogen and phosphorus were limiting factors that affect oil degradation.
出处 《环境科学与技术》 CAS CSCD 北大核心 2009年第1期19-22,44,共5页 Environmental Science & Technology
关键词 PCR测序 营养平衡 疏水性降解菌 PCR sequencing nutrient balance hydrophobic hydrocarbon degrading bacteria
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