摘要
茵陈蒿是常用的保肝中药，具有提高机体免疫功能和抗御肿瘤的作用。本研究利用茵陈蒿可能具有抗突变作用，对强致癌剂ＡＦＢ１进行Ａｍｅｓ试验、小鼠骨髓细胞的遗传毒理学实验，以评价茵陈蒿的抗突变能力。结果表明：１茵陈蒿Ａｍｅｓ试验为阴性。在ＡＦＢ１（５μｇ／皿）培养基中加入０１ｍｌ浓度分别为０１２５、０２５、０５、１、２ｇ／ｍｌ的茵陈蒿煎剂，ＴＡ９８和ＴＡ１００的回变菌落抑制率达２２９－５４３６，差异极其显著（Ｐ＜００１）。２茵陈蒿煎剂对ＡＦＢ１诱发的小鼠骨髓嗜多染红细胞微核率、染色体畸变率和姊妹染色单体交换率的增高有明显的拮抗作用（Ｐ＜０．０１），且具有明显的剂量———反应关系。本结果提示茵陈蒿可能对癌症预防与治疗有积极的意义。
Objective To inhibit the mutagenicity and genetic damages of mouse marrow cells induced by Aflatoxin B 1 (AFB 1), Artemisia capillaris Thumb(ACT) had been used to evaluate the possibility of antimutagenicity. Methods The tests of Ames test, micronucleus, chromasome aberration and sister chromatid exchanges of mouse bone marrow cells were used to detect the mutagenicity and antimutagenicity of ACT and AFB 1. Results As expected, the mutagenicity of ACT was negative. Put 0.1 ml ACT extract (the concerntration was 0.125, 0.25, 0.5, 1.0 and 2.0 g/ml) to culture medium of AFB 1 (5μg/plate), the inhibition rates of TA98 and TA100 were 22.9-54.36, the difference was very remarkable ((P<0.01). The inhibition effects of ACT to micronuclei, chromosome aberration and sister chromatid exchanges indued by AFB 1 were very remarkble too, and the relationship of dosereaction was very evident. Conclusion These results showed that ACT had very strong inhibition to the mutagenicity and genetic damages induced by AFB 1 and might have the function to repair the DNA damages, which suggested that ACT would have greater meaning to prevent or treat cancers.
出处
《癌变．畸变．突变》
CAS
CSCD
1998年第1期35-38,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
茵陈蒿
微核
染色体畸变
AMES试验
抗肿瘤作用
Artemisia capillaris Thumb
Aflatoxin B 1
Micronucleus
Chromasome abbrration
Sister chromatd exchanges
Ames test
