摘要
目的探讨胚胎发育信号通路Sonic hedgehog（SHH）在胰腺癌组织中的表达及其与表皮生长因子受体（EGFR）的关系。方法逆转录-聚合酶链反应（RT-PCR）和Western blot法分别检测胰腺癌组织及癌旁组织中SHH和EGFR的mRNA和蛋白表达。结果SHH mRNA和蛋白在胰腺癌组织中的阳性率分别为81．6％和79．6％，与癌旁组织比较，差异有统计学意义（P〈0．05）。EGFR mRNA和蛋白在胰腺癌组织中的阳性率均为73．5％，与癌旁组织比较，差异有统计学意义（P〈0．05）。SHH和EGFR蛋白表达与年龄、肿瘤大小、组织学类型和肿瘤部位等病理因素均无明显相关（P〉0．05），而在不同淋巴结转移状况和TNM分期的病例组中，两者表达差异有统计学意义（P〈0．05）。配对资料的Spearman相关分析显示，SHH表达与EGFR表达呈正相关（r=0．232，P〈0．05）。结论SHH和EGFR信号通路在胰腺癌组织中呈活化状态，两者之间的相互作用对胰腺癌的发生发展可能有重要影响。
Objective To investigate the expression of Sonic hedgehog （SHH） and EGFR signaling pathways in human pancreatic cancer. Methods Reverse transcription-polymerase chain reaction （RT-PCR） and Western blot assay were used to detect the mRNA and protein expression of SHH and EGFR in human pancreatic cancer tissue and normal tissues adjacent to cancer. Results The SHH mRNA and protein expression was detectable in 81.6% and 79.6% of pancreatic cancer, respectively. The EGFR mRNA and protein expression was both 73.5% in pancreatic cancer tissues. These was significant difference from that of normal tissue adjacent to cancer （ P 〈 0.05 ）. The expression of SHH and EGFR protein in pancreatic cancer had no correlation with age, tumor size, pathological type and tumor site （P 〉 0.05 ） , hut had a relationship with lymph node metastasis and TNM stage （ P 〈 0.05 ）. The relationship between SHH and EGFR protein was positive （r = 0.232 ,P 〈 0.05 ）. Conclusion The SHH and EGFR signaling pathways were active in human pancreatic cancer. The crosstalk between these pathways may play an important role in the carcinogenesis and development of pancreatic carcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第1期55-57,共3页
Chinese Journal of Experimental Surgery
