摘要
（目的）分析肺癌组织多药耐药基因表达情况及其临床意义。〔方法〕运用RT－PCR方法对51例手术切除肺癌组织和相应的癌周正常肺组织进行MDRI mRNA水平检测。（结果）MDRI mRNA在正常肺组织和肺癌组织中表达阳性率分别为 13、 7％（7／51）和 43． 1％（22／51），两者差异显著（P＜0.01）。MDR1 mRNA表达与肿瘤病理类型、组织分化、TNM分期等均无明显的相关性（P＞0. 05）。〔结论〕肺组织细胞在恶变过程 MDR1 mRNA水平表达明显增加，提示 MDR1基因是肺癌标志之一，同时在肺癌先天也耐药机制中占有重要的作用。
(Purpose) To study the expression of MDR1 gene in patients with lung cancer and its clincal significance. (Methods)Detection of multidrug resistance gene in 51 lung cancer specimens and corresponding normal lung tissue specmens from the neighbourhood of the cancer focuses by reverse transcription-polymerase chain reaction (RT- PCR ). These cases did not undergo any anti -cancer treatment. (Results) 7 of the 51 nortnal tissue(1 3. 7 % ) and 22 of the 51 tumor specimens(43. 1 % ) expressed MDR1 gene (P＜0. 01 ).No correlations were noted among MDR1 gene expression, histological differentiation, pathological type, and TNM stage.(Conclusions) These results suggest that the level of MDR1 gene expression in lung cells is incresaed as cells progress from the normal to the transformed state. So it might be a tumor marker of lung caner. Moreover, it is a importment factor of drug resistance mechanism of lung cancer.
