摘要
目的:探讨心肌脂肪酸结合蛋白(Heart fatty-acid-binding protein,H-FABP)诊断早期心肌梗死的死后诊断的稳定性。方法:建立兔急性心肌缺血模型,应用免疫组化和图像分析技术,检测死后不同时间缺血心肌细胞内H-FABP脱失面积变化规律。结果:正常心肌组织4℃放置1～2d H-FABP染色均匀未见明显脱失,放置3d以上,可见H-FABP脱失,且随放置时间的延长,脱失面积逐步增大。缺血心肌组织随放置时间的延长,脱失面积逐步增大,图像分析结果显示4d以内缺血心肌组织胞浆内H-FABP的脱失面积与正常对照组比较差异有统计学意义(P<0.01),放置超过5d以上,其脱失面积与正常对照组比较差异无统计学意义。结论:H-FABP在心肌梗死死后诊断的稳定性一般,受自溶的影响较小可用于死后4℃放置的4d内尸体的检测,在法医学实践中具有一定的实用价值。
Objective： To investigate the stability of heart fatty-acid-binding protein（H-FABP）for the postmortem diagnosis of early myocardial infraction.Methods： Rabbit model of myocardial ischemia was induced by ligating the anterior branch of the left coronary artery.The areas of H-FABP depletion in the ischemic myocardial cells were studied by immunohistochemistry,image analysis technique and statistical system. Results： The specimens of normal myocardium kept at 4℃ for l to 2 days showed homogenous brown reactions for H-FABP. The depletion of H-FABP became evident when these specimens were kept at 4℃ for 3 days after death,and the depletion area increased with the lapse of postmortem interval. The depletion area of H-FABP in ischemic myocardial tissue also increased with the lapse of postmortem interval. The results of image analysis indicated that the depletion area of H-FABP in ischemic myocardial tissue within 4 d were significantly different from that in the normal control group（P〈0.01 ）,while within 5 d the depletion area of H-FABP in the two groups was not significantly different（P〉0.05）. Conclusion： The H-FABP shows medium postmortem stability,and small influenced by autolysis,which is suitable for detection in corpses at 4℃ 4 days after death. H-FABP as a marker for postmortem diagnosis of AMI has some practical value in forensic medicine.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第12期1488-1491,共4页
Journal of Chongqing Medical University
关键词
早期心肌梗死
心肌脂肪酸结合蛋白
稳定性
Early myocardial infraction
Heart fatty-acid-binding protein
Stability
