摘要
简要介绍了ＣＥ在ＤＮＡ、糖和离子分析中的应用。ＤＮＡ分析包括分离原理、方法及检测手段，碱基、核苷和核苷酸分析，纯度检测和微量制备，ｓｓＤＮＡ和ｄｓＤＮＡ分析，基因突变检测及ＤＮＡ序列分析等。糖分析包括衍生化技术、检测方法、所用模式及糖复合物的分析等。离子分析包括阴离子分析原理、检测方法、影响因素、应用及阳离子分析等。
A review with 87 references on the application and new development of CE in DNA analysis, carbohydrate analysis and capillary ion analysis is presented. The DNA analysis included determinations of neucleotide, nucleoside and bases by CZE and MECC. It also includes dsDNA, PCR products analysis, purity control of oligonucleotide and DNA sequencing by CGE. Sometimes LIF detector needs to be introduced into DNA analysis. The carbohydrate analysis includes the methods for chemical derivatization and direct determination of carbohydrates by different CE modes. The capillary ion analysis includes the principle and determination of anions and cations.
出处
《色谱》
CAS
CSCD
北大核心
1998年第1期38-43,共6页
Chinese Journal of Chromatography
基金
国家自然科学基金
关键词
毛细管电泳
DNA
糖
离子分析
capillary eletrophoresis, DNA analysis, carbohydrate analysis, capillary ion analysis
