摘要
调查了上海市部分规模化屠宰场生猪屠宰产品中戊型肝炎病毒的携带状况。从4个屠宰场采集60份猪胆汁、60份猪肝脏、60份猪脾脏、60份猪肠系膜淋巴结、160份猪粪便及61份猪血清样品。利用巢式简并引物和RT-PCR技术,检测样品中HEVRNA,并对阳性扩增子进行克隆测序,序列用生物信息学软件分析。结果表明:样品戊型肝炎病毒RNA阳性率为1.08%(5/461),其中胆汁检出率为3.3%(2/60)、粪样为0.6%(1/160)、血清3.2%(2/61)、肝脏、脾脏和肠系膜淋巴结未检测到HEVRNA。序列系统进化分析结果显示,5株HEV中3株为基因3型HEV,同源性为90.7%～98.6%;2株为基因4型,同源性为94.9%。结果证实屠宰场生猪携带的HEV主要存在于胆汁和血清中。因此提示,屠宰场在屠宰时要妥善处理生猪血清和胆汁以防污染其他屠宰产品。研究在屠宰生猪中检测到基因3型HEVRNA。结果提示,至少在较为局限的群体中基因3型和4型病毒可以共存。
To investigate the presence of swine hepatitis E virus （HEV） of several large-scale slaughterhouses in scales in Shanghai. The bile, liver, spleen and lymphonodi mesenterici of 60 swine were collected respectively, 160 swine fecal specimens and 61 random sera were collected randomly in four slaughterhouses. The HEV RNA were detected by nested RT-PCR, the HEV positive PCR products were sequenced and sequence analysis was then performed by using bio-software. The positive rate of tIEV was 1.08 % （5/461）. Sequence analysis showed that the 5 isolates included three genotype 3 HEV strains and two genotype 4 HEV strains. The three genotype 3 HEV isolates shared 90.7%-98.6% identity with each other, the two genotype 4 isolates shared 94.9% identity to each other. Conclusions： The presence rate of HEV was various in different organs of the same carcass. Bile and serum were the most sensitire organ to HEV. This suggested that the slaughterhouses should pay more attention to slaughter products for avoiding HEV contamination. What＇s more, in the investigation, it was the first time that genotype 3 HEV strains were detected in swine of slaughter-houses, this confirmed that gcnotype 3 HEV had been prevalent in mainland of China.
出处
《上海交通大学学报（农业科学版）》
2008年第6期511-515,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科委技术标准专项(06DZ05132)
上海科技攻关计划重点项目(063919121)
