摘要
【目的】改进大鼠心肌缺血后处理模型的制备方法,提高制模成功率与效率。【方法】健康雄性SD大鼠80只,随机分为传统心肌缺血/再灌组(A组,n=20),改良心肌缺血/再灌组(B组,n=20),传统缺血后处理组(C组,n=20),改良缺血后处理组(D组,n=20)。观察各组制模成功率与效率,并从血流动力学、心电图、心肌缺血与梗死面积、心肌酶学、病理学等方面对改进的模型进行评估。【结果】A、B、C、D组的制模成功率分别为80%、90%、75%、85%,传统组与改良组无差异(P>0.05)。传统组每只模型从麻醉到关胸约45 min,改良组约10 min;传统组呼吸机使用时间165 min,改良组为3 min。改良组造模时间缩短,效率高于传统组。血流动力学、心肌酶学、病理学等各种方式均证实改良组造模结果成功。【结论】改良大鼠心肌缺血后处理制模方式保留了传统造模方式成功率高的优点,同时提高了造模效率,造模结果稳定可靠。
[Objective] To introduce the more effective method to establish rat models of myocardial ischemia postconditioning. [Methods] Eighty adult healthy SD rats were divided into four groups at random： traditional myo- cardial ischemia/reperfusion group （A group, n = 20）, improved myocardial ischemia/reperfusion group （B group, n= 20）, traditional myocardial ischemia postconditioning group（C group, n = 20） and improved myocardial ischemia postconditioning group （D group, n = 20）. The successful rate and the effectiveness of each group were observed. The hemodynamics, electrocardiogram, Evans blue and TTC staining, myocardial enzyme spectrum and histology resuhs were used for evaluating the modified model. [Results] The successful rate of A, B, C, D groups were 80%, 90%, 75% and 85%, respectively, and the times from anesthesia to close the chest in each group were 45min, 10min, 45min and 10min, respectively. The artificial ventilation times were 165min, 3min, 165min, and 3rain, respectively. The hemodynamic trend of each group was similar. Electrocardiogram, Evans blue and TTC staining, myocardial enzyme spectrum, and histology all testified that the models of modified method were successful. [Conclusion] The method of establishing rat model of myocardial isehemia postconditioning is simple, convenient and reliable, with high successful rate and higher effectiveness.
出处
《医学临床研究》
CAS
2008年第12期2155-2158,共4页
Journal of Clinical Research
关键词
心肌缺血
疾病模型
动物
大鼠
myocardial ischemia
disease models,animal
rats
