摘要
本文应用ＡｐａＩ、ＢａｍＨＩ、ＢｃｌＩ、ＢｇｌＩ、ＣｌａＩ、ＥｃｏＲＩ、ＥｃｏＲＶ、ＨｉｎｄＩＩ、ＰｓｔＩ、ＰｖｕＩＩ、ＳａｃＩ、ＳｃａＩ和ＸｂａＩ等１３种限制性内切酶对板齿鼠线粒体ＤＮＡ（ｍｔＤＮＡ）进行限制性片段长度多态（ＲＦＬＰ）分析，并用双酶解法构建其限制性内切酶图谱。结果表明板齿鼠存在３种ｍｔＤＮＡ单倍型，可通过限制酶ＰｖｕＩＩ、ＨｉｎｄＩＩ和ＡｐａＩ区分，呈现ＤＮＡ多态性和种内遗传变异。与小家鼠、褐家鼠ｍｔＤＮＡ限制性片段的数据相比较，板齿鼠和这两种鼠ｍｔＤＮＡ存在明显差异。板齿鼠ｍｔＤＮＡ限制性内切酶图谱的建立，为进一步系统研究鼠科动物的遗传分化提供了依据。
? Mitochondrial DNA (mtDNA) samples from 9 individuals of Bandicota indica captured from 3 different sites in Guangdong province were digested individually with each of the following 13 restriction endonucleases Apa I、Bam HI、Bcl I、Bgl I、Cla I、Eco RI、Eco RV、Hind III、Pst I、Pvu II、Sac I、Sca I and Xba I for RFLP analysis. All 31 fragements and 16 restriction morphs were recognized , the samples from different sites were grouped into three mtDNA halotypes which could be distinguished by Pvu II , Hind III and Apa I,indicating the mtDNA polymorphism within B.indica . Restriction map was made by the doubleenzyme digestion method and it would be very helpful to the study of the phylogentic relationships among different species in the Murida. There were significant differences in RFLP of mtDNA among B.indica , rat and mouse, B.indica might have closer relationship with rat than that with mouse.
出处
《兽类学报》
CAS
CSCD
北大核心
1998年第1期54-59,共6页
Acta Theriologica Sinica
基金
中国科学院广州分院
广东省科学院院长基金
关键词
板齿鼠
线粒体DNA
限制性内切酶
图谱
DNA多态性
Bandicota indica, Mitochondrial DNA, Restriction map, RFLP(Restriction fragment length polymorphism)
