摘要
两阶段珠到珠转移方法（包含最初8h的间歇搅拌和接下来的连续搅拌阶段）可以实现Vero细胞的扩大培养，按照新老载体2：1的比例放大，载体空载率能从最初的66．7％降到3．5～6．3％。在间歇搅拌阶段，转速对新载体建桥率没有显著影响，但在连续搅拌阶段，60r／min转速仅能使载体空载率降低到28．9％，甚至比35r／min连续搅拌产生的空载率还高8％。在新老载体1：1放大过程中，5eg／mL的胰酶不能促进细胞在载体Cytodex-3间的转移，最后的载体空载率仍高达10．8％。这些研究结果为Vero细胞通过珠到珠转移方式在其他系统或以更大扩增倍数进行的放大培养过程提供了重要信息。
A two-stage bead-to-bead transfer of Vero cells was set up, including the first 8 h intermittent agitation and the following continuous agitation stages. In 2 ： 1 expansion （ the ratio of fresh and confluent microcarriers）, the percentage of bare microcarriers decreased quickly from 66.7% to 3.5--6.3%. Stirring speed had no significant influence on the percentage of bridged fresh microcarriers in the intermittent agitation stage, but a high stirring speed 60 r/min could only make the percentage of bare microcarriers decrease to 28.9 %, 8 % higher than that coming from continuous agitation at 35 r/min. In 1 ： 1 expan- sion, 5 μg/mL trypsin couldn＇t improve the cell bead-to-bead transfer between cytodex-3, and the percentage of bare microcarriers still remained at 10.8%. These observations will provide valuable information on the bead-to-bead transfer of Vero cells in other systems and at a larger scale.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2008年第6期801-804,共4页
Journal of Jilin Agricultural University
基金
上海市科委项目(053919142)
