摘要
副溶血性弧菌(Vibrio parahaemololyticus,Vp)是我国微生物食源性疾病的主要病因,且是海水养殖业中的常见病原菌,造成巨大的经济损失。本研究制备副溶血弧菌免疫原,并以5×107、5×108CFU/ml两种剂量分别免疫母鸡,获得特异性的抗副溶血弧菌IgY;并建立ELISA方法检测免疫鸡蛋中的抗体效价,高剂量免疫组的抗体效价可达1:57600,低剂量免疫组的抗体效价达1:28800;低剂量组抗体效价可维持250d,高剂量组抗体效价可维持300d以上。
Vibrio parahaemololyticus is the main cause of foodborne disease, and it is also a common pathogen detected from seafood. Vibrio parashaemoloyticus would cause enormous loss when fish and shrimp and so on are infected by it. In this study, laying hens were divided into two groups, and were immunized with two different doses of prepared antigen. The low dose group of hens was immunized with 5 × 10^7 CFU/ml, and the high dose group of hens was immunized with 5 × 10^8 CFU/ml Vibrio parahaemololyticus. ELISA was established to detect the antibody activity. The results demonstrated that the highest titer of IgY against Vibrio parahaemololyticus in the high and low dose group could reach 1：57600 and 1：28800, respectively. The titer of IgY produced by the low dose group could maintain for 250 days, but the titer of IgY produced by the high dose group could maintain for more than 300 days.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第12期469-472,共4页
Food Science
关键词
副溶血性弧菌
IGY
制备
活性检测
Vibrioparahaemololyticus
IgY
production
antibody activity
