摘要
庞大的瘤胃微生物群系之间存在着共生关系,影响着宿主的代谢,是反刍动物营养学的研究热点之一。通过基于16S rRNA的分子生物学方法,如探针法、实时定量PCR法、DGGE/TGGE、RAPD和RFLP技术等研究瘤胃微生物多样性及组成结构,应用宏基因组学如建立YAC文库、BAC文库等研究方法对瘤胃微生物功能特征进行更深入的研究,实现改善反刍动物乳、肉产品品质的目的。
There is a symbiotic relationship between the huge ruminant microflora, which plays a crucial role in the metabolic mechanism of ruminant animal. This makes the ruminant microflora as a hotspot. Using methods of molecular biology such as probe methods, Real-time PCR DGGE/TGGE, RAPD and RFLP to study ruminant microbial ecology and metagenomic methods including YAC library and BAC library to study ruminant microflora functionality would attain the aim to approve the quality of milk and meat, meanwhile to explore the abundant resources of ruminant microflora.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第12期1961-1965,共5页
Microbiology China
基金
国家“十一五”科技攻关奶业重大专项(No.2006BAD04A00,No.2006BAD12B08)
动物营养学国家重点实验室自主研究课题[No.2004DA12584(团)0801]
关键词
瘤胃微生物
多样性
功能性
研究方法
Ruminant microflora, Diversity, Functionality, Methodology
