摘要
目的:研究结核分枝杆菌耐链霉素和乙胺丁醇的rpsL和emb B基因突变情况,探讨耐药基因突变与耐药性的关系。方法:通过传统药敏实验和聚合酶链反应(PCR)--单链构象多态性(SSCP)技术初步鉴定62株临床分离株的药敏和rps L、emb B基因。结果:与结核菌标准株H37Rv对照,分析30例TB菌耐链霉素(SM)的rps L基因,发现其突变率为70.0%(21/30),分析29例耐乙胺丁醇(EMB)的emb B基因,该基因的突变率为65.5%(19/29)。结论:部分结核分枝杆菌耐SM和EMB是由于其rps L、emb B基因突变所致,PCR-SSCP银染技术可能成为测定部分结核分枝杆菌耐药的简便、快速的方法。
Objective： To study the mutation ofrps L and emb B genes in mycobacterium tuberculosis isolates, and investigate the relationship between drug-resistance gene mutations and drug-resistance in mycobacterium tuberculosis. Methods： The drug-sensitivity of 62 clinical isolates of mycobacterium species were identified by the traditional method, and then the mutation of their emb B and rps L genes were analyzed with PCR-SSCP. Mycobacterium tuberculosis strain H37RV was used as a control. Results： The mutational frequency of rps L gene in 30 SM - resistant mycobacterium tuberculosis was 70.0 % （21/30）. The mutational frequency of emb B gene in 29 EMB- resistant isolates was 65.5 % （19/29）. Conclusion： SM and EMB resistances were due to mutations ofrps L and emb B genes in some mycobacterium tuberculosis isolates. PCR-SSCP method might become a simple and rapid diagnostic test for genotypes of SM and EMB resistant mycobacterium tuberculosis.
出处
《现代生物医学进展》
CAS
2008年第12期2272-2274,共3页
Progress in Modern Biomedicine
基金
新疆结核病耐药机制及临床应用的研究(兵团医药卫生专项
04GG23)
关键词
结核分枝杆菌
链霉素
乙胺丁醇
耐药性
Mycobacterium tuberculosis
Streptomycin
Ethambutol
Drug resistance
