摘要
采用重叠区扩增法人工合成了大肠杆菌偏爱密码子的猪PR-39基因片段,并克隆至pMD18-T中,经测序确认正确后,与pET-Trx构建了串联表达载体pET-Trx-(PR-39)3,并在大肠杆菌BL21(DE3)中诱导获得表达。为PR-39的进一步开发与应用打下了基础。
Gene RP-39 from pig with the partiality of E coli was synthesized by the method of gene splicing by overlap extension. The fragment was cloned into pMD18-T and sequenced. Three copies of gene RP-39 were combinated into express vector pET-Trx. Then pET-Trx-（PR-39）3 was transformed into E coli BL21 （DE3） and RP-39 was inducted which established good foundation for the future development.
出处
《中国畜牧兽医》
CAS
2008年第12期56-59,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家“863”计划现代农业技术领域重大项目(2006AA10A211)
关键词
基因合成
PR-39
串联
载体构建
gene synthesis PR-39 tandem repeats
construction of expression vector
