摘要
目的探讨应用血管内皮细胞生长因子(VEGF)与肿瘤坏死因子受体(TNFR)联合治疗股骨头坏死的治疗效果。方法新西兰兔30只,26只兔耳缘静脉注射马血清,臀肌注射醋酸泼尼松龙,制造激素性股骨头坏死动物模型。动物随机分为4组:A组:VEGF/TNFR治疗组;B组:VEGF治疗组;C组:对照组;D组:正常对照组。治疗组骨穿刺针行经皮注射血管内皮细胞生长因子脂质体及TNFR至股骨头。于治疗后,应用ELISA法测血清中TNF-α浓度的变化,病理组织学观察股骨头骨组织、骨髓造血组织及血管的变化。结果VEGF/TNFR治疗组,TNF-α浓度及活性明显降低,HE染色结果:VEGF/TNFR治疗组可见软骨细胞修复性增生,骨髓腔造血组织出现增生,栓塞的血管旁边出现新生的血管。结论VEGF/TNFR联合治疗股骨头坏死能促进新血管生成及骨修复。
Objective To evaluate the therapeutic effect of vascular endothelial growth factor （VEGF） and tumor necrosis factor receptor （TNFR） on avascular necrosis of the femoral head in rabbits. Methods Avascular necrosis of the femoral head was induced in 26 New Zealand white rabbits by injections of horse serum and prednisolone. The rabbits were then divided into VEGF/TNFR treatment group, VEGF treatment group, and untreated model group, with another 4 normal rabbits as the normal control group. In the two treatment groups, the therapeutic agents were injected percutaneously into the femoral head. Enzyme-linked immunosorbent assay was performed to determine the concentration of TNF-α in rabbit serum followed by pathological examination of the changes in the bone tissues, bone marrow hematopoietic tissue and the blood vessels in the femoral head. Results Compared with the model group, the rabbits with both VEGF and TNFR treatment showed decreased serum concentration of TNF-α with obvious new vessel formation, decreased empty bone lacunae in the femoral head and hematopoietic tissue proliferation in the bone marrow cavity. Conclusion Percutaneous injection of VEGF and TNFR into the femoral head can significantly enhance bone tissue angiogenesis and ameliorate osteonecrosis in rabbits with experimental femoral head necrosis.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第12期2177-2179,2182,共4页
Journal of Southern Medical University
基金
广东省自然科学基金(06301184)
广州市科技计划(2003Z2-E0171)
