摘要
目的研究N-(4-羟基苯基)维生素甲酰胺(4HPR)对人宫颈癌细胞凋亡的影响。方法应用光学显微镜、激光共聚焦显微镜、电子显微镜、荧光显微镜从细胞形态学方面观察4HPR对人宫颈癌细胞凋亡的影响;应用流式细胞仪、细胞DNA琼脂糖凝胶电泳法分析4HPR诱导人宫颈癌细胞凋亡的细胞特征、生物化学特征及凋亡细胞百分率。结果电子显微镜和激光共聚焦显微镜镜下可见细胞固缩,核膜扭曲,核染色体聚集成块并靠近核膜等凋亡细胞特征;光学显微镜和荧光显微镜下可见凋亡小体;细胞DNA被降解,在琼脂糖凝胶电泳中呈现典型的"阶梯状"图谱;流式细胞仪检测结果显示二倍体核型的特征,在DNA直方图上,G1峰左侧出现亚二倍体细胞群的峰型;凋亡百分率结果显示4HPR可诱导宫颈癌细胞凋亡,且呈时间和浓度依赖性(P<0.01)。结论4HPR可诱导人宫颈癌细胞凋亡,且呈时间和浓度依赖性。
Objective To investigate the effects of N-（4-hydroxyphengl） retinamide （4HPR） on apoptosis in Hela cells. Methods Morphologic changes of Hela cells induced by 4HPR were detected by hght microscopy, laser focusing, fluorescent and electron microscopy. The morphologic and biochemical features and the percentage of apoptosis in Hela cells were determined by flow cytometry（FCM） and DNA agarose gel electropboresis. Results It was found that Hela cells had obvious morphological changes, such as cell pykermis, nuclear membrane twist, nuclear chromomocre assembled to lump and closed to membrane under laser focusing and electron microscopy. Apeptotic bodies could be found under light microscopy and fluorescent microscopy. Typical DNA ladder appeared in 4HPR-treated cells in DNA agarose gel electmphoresis. Detected by flow cytometry, the sub-diploid nuclear peak appeared to the left of the G1 peak, and the diploid cell peak was reduced. The apoptotic percentage of Hela ceils was positively correlated with the concentration and time of the 4HPR action（ P 〈 0.01）. Conclusion 4HPR could induce cervical cancer cell apoptosis in concentration-and time-dependent manners.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第12期1120-1123,共4页
Journal of Shandong University:Health Sciences
基金
江苏大学高级人才基金项目(JDG2004008)
