摘要
目的探讨CDH1基因3′-UTR +54C/T SNP与中国北方人群肺癌遗传易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法检测194名肺癌患者和223名健康对照组3′-UTR +54C/T SNP的基因型。结果肺癌患者组吸烟个体比例明显高于对照组,吸烟可增加肺癌的发病风险(OR=3.03,95%CI=2.03～4.54)。肺癌患者组C等位基因频率(85.6%)显著高于对照组(76.9%),两组相比差异有统计学意义(χ2=10.09,P=0.00);肺癌患者组与对照组T/T、T/C和C/C基因型频率分别为1.0%、26.8%、72.2%和4.0%、38.1%、57.8%,与T/T或T/C基因型相比,携带C/C基因型可显著增加肺癌的发病风险(OR=1.89,95%CI=1.25～2.85)。结论CDH1基因3′-UTR +54C/C基因型可能是中国北方人群肺癌发病的潜在危险因素。
Objective This study was designed to investigate the correlation between CDHI 3′-UTR ＋ 54 locus C/T SNP and susceptibility to lung cancer in Northern Chinese population. Methods 3′-UTR ＋ 54 locus C/T SNP was genotyped by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） analysis in 194 lung cancer patients and 223 healthy controls. Results The number of the smokers in lung cancer patients was significantly higher than that in healthy controls. Smoking may increase the risk of developing lung cancer [odds ratio （OR） = 3. 03, 95%（CI） = 2. 03-4. 54]. The C allelotype frequence of CDH1 in lung cancer patients was 85.6%, which was significantly higher than that in healthy controls （χ^2 = 10. 09, P = 0. 00）. The T/T, T/C and C/C genotype frequencies of lung cancer patients and healthy controls were 1.0%, 26. 8%, 72.2% and 4. 0%, 38.1%, 57. 8%, respectively. Compared with individuals with T/T or T/C genotype, individuals with C/C genotype had significantly higher risk in developing lung cancer （OR = 1.89,95%CI = 1.25-2. 85）. Conclusion The C/C genotype of CDH1 3′-UTR ＋ 54 locus might be a potential risk for lung cancer development in Northern Chinese population.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2008年第12期900-903,共4页
Cancer Research on Prevention and Treatment
基金
河北省科技厅科技支撑课题资助项目(08276101D-41)
