摘要
目的观察A型肉毒毒素(BTX-A)在大鼠化学性内脏痛中的止痛效应及对肠壁P物质(SP)和降钙素基因相关肽(CGRP)表达的影响。方法8周Wistar雄性大鼠48只,随机均分为A、B、C、D组,分别腹腔注射生理盐水2 mL、含2 U、4 U、6 UBTX-A的生理盐水2 mL,1周、4周后各组分别抽取6只进行腹腔注射乙酸的扭体实验后,留取肠道标本行肠壁SP、CGRP免疫组化测定。结果1周时,C、D组疼痛积分和肠壁CGRP表达均少于A组(P<0.05),各BTX-A组肠壁(除B组小肠黏膜层)SP较A组均明显减少(P<0.05);4周时,B、C、D组的疼痛积分和肠壁SP明显少于A组(P<0.05),各组各部位肠壁(除B组小肠肌层)CGRP均少于A组(P<0.05)。结论腹腔注射BTX-A能减轻乙酸所致的大鼠内脏痛的疼痛反应,并能减少肠壁SP、CGRP的表达。
Objective To investigate the effect of botulinum toxin type A （BTX-A） on relieving chemical visceral pain and the expression of intestinal SP and CGRP in rats. Methods Forty-eight male Wistar rats of postnatal eight weeks were divided into four groups： BTX-A （2 U, 4 U and 6 U） or normal saline （2 mL） were injected intraperitoneally of B, C, D or A group. Six rats of every group were challenged with acetic acid intraperitoneal injection 1 week and 4 weeks post-BTX-A injection. After abdominal writhing behaviors were monitored, the intestinal samples were get and immunohistochemical stained for SP and CGRP examination. Results At the end of one week, writhing test scores and intestinal CGRP expression of group C and D were decreased significantly compared with group A （P 〈 0.05）. The expression of SP of all BTX-A pretreated groups （ except in small intestinal mucosal layer of group B） showed a significant decrease （P 〈 0.05）. Four weeks later, the writhing test scores and the expression of SP and CGRP （except CGRP in small intestinal muscular layer of group B） in BTX-A pretreated groups all showed significant difference compared with those of group A（P 〈 0.05）. Conclusion Injection of BTX-A intraperitoneally significantly reduces acetic acid-induced nociceptive behaviors and inhibits the expression of SP and CGRP in intestinal walls.
出处
《胃肠病学和肝病学杂志》
CAS
2008年第12期1023-1025,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
A型肉毒毒素
内脏痛
P物质
降钙素基因相关肽
Botulinum toxin A
Visceral pain
Substance P
Calcitonin gene-related peptide
