摘要
目的通过对云南非铀矿山矿工血清IL-2、IL-6和TNF-α浓度及基因多态性位点的检测，对氡致非铀矿山矿工机体生物效应进行初步探讨。方法血清中IL-2、IL-6和TNF-α的浓度测定采用双抗夹心ABC—ELISA法。TNF-α（-308，G→A）检测基因型采用基因体外扩增限制性片段长度多态性分析方法，IL-2（-330，T→G）基因型检测采用PCR体外扩增后测序的方法。结果矿工组与对照组比较，年龄（F=1．07，P〉0．05），工龄（F=0．40，P〉0．05），血清IL-2浓度（F=0．71，P〉0．05），血清IL-6浓度（F=1．09，P〉0．05），血清TNF-α浓度（F=0．95，P〉0．05），IL-2基因型频度（χ^2=0．02，P〉0．05）和TNF-α基因型频度（χ^2=2．21，P〉0．05），差异均无统计学意义。结论云南东川非铀矿山矿工血清IL-2、IL-6和TNF-α平均浓度有下降的趋势，TNF-α（-308，A／A）基因型频度有增高的趋势。
Objective To study biologic effect of high radon exposure in non-uranium miners by measurements of serum levels of IL-2, IL-6 and TNF-α and study on their gene polymorphism. Methods Serum levels of IL-2, IL-6 and TNF-α in the miners selected from a Yunnan-based copper mine were measured by ABC- ELISA. TNF-α （ -308, G→A） genotypes were identified by RFLP-PCR, IL-2 （ -330, T→G） genotypes by sequence analysis. Results Compared the miners with its control group, there were no statistical significance of the concentrations of serum IL-2 （ F = 0.71, P 〉 0.05）, IL-6 （ F = 1.09, P 〉 0.05） and TNF-α （ F = 0.95, P 〉 0.05）. Frequencies of IL-2 （ - 330, T→G） genotypes （ χ^2 = 0.02, P 〉 0.05） and TNF-α （ - 308, G→A） genotype （χ^2 = 2.21, P 〉 0.05） were shown no statistical significance too. Conclusions Compared with the control group, concentrations of serum IL-2, IL-6 and TNF-α in miners working in the copper mine was lower, frequencies of genotypes of TNF-α （ -308, A/A） was higher in the miners. But all the differences were not statistical significant.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2008年第6期576-578,共3页
Chinese Journal of Radiological Medicine and Protection
