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不同浓度离子霉素对猪体外成熟卵母细胞孤雌发育的影响 被引量:1

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摘要 试验探讨了不同浓度的离子霉素结合6-二甲氨基嘌呤激活猪体外成熟卵母细胞后对其孤雌发育的影响。体外成熟卵母细胞分别用10、15、20、25mol/L的离子霉素处理40min,再用2mmol/L的6-DMAP处理8h,各组的卵裂率分别是:(13.11±6.75)%,(22.31±14.18)%,(26.93±24.53)%和(26.72±19.75)%,囊胚率分别是2.26±1.03)%,(2.39±2.41)%,(3.32±3.95)%和(11.76±10.76)%,各组之间差异不显著(P>0.05)。结果表明:用10~25mol/L离子霉素和6-DMAP联合激活猪体外成熟卵母细胞,对其孤雌发育能力有一定影响,但效果不显著;猪卵母细胞采用离子霉素激活的最佳方案是用25mol/L处理40min,再用浓度为2mmol/L的6-DMAP处理8h。
出处 《现代畜牧兽医》 2008年第12期12-13,共2页 Modern Journal of Animal Husbandry and Veterinary Medicine
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