摘要
利用高效凝胶渗透色谱(HPGPC)测定芦荟浓缩液和芦荟粉中多糖的纯度和相对分子质量,使用1-苯基-3-甲基-5-吡唑啉酮(PMP)作为柱前衍生试剂分别对样品和其水解产物进行衍生,结合高效液相色谱(HPLC)分离和电喷雾-离子阱质谱(ESI-MS)检测分析其单糖组成。结果表明:组成芦荟中多糖的单糖主要是甘露糖和葡萄糖。
High performance gel permeation chromatography （HPGPC） was applied to the determination of purity and relative molecular mass of polysaccharides in concentrate liquor and powder of aloe. The aloe sample （in liquor or powder state） or its hydrolysis product was pre-colurnn derivatized with 1-phenyl-3-methyl-5-pyrazolone （PMP）, and the compositional monosaccharides were separated and determined by hyphenation of HPLC with electron spray ionization-mass spectroscopy （ESI-MS）. It was found that marmose and glucose were the main compositional monosaccharides of polysaccharides in aloc.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2008年第12期1133-1136,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
关键词
芦荟多糖
单糖
高效凝胶渗透色谱
高效液相色谱
电喷雾-离子阱质谱
柱前衍生
1-苯基-3-甲基-5-吡唑啉酮
Aloe polysaccharide
Monosaccharides
High performance gel permeation chromatography（HPGPC）
High-performance liquid chromatography （HPLC）~ Electron spray ionization-mass spectroscopy（ESI-MS）
Precolumn derivatization
1-Phenyl-3-methyl-5-pyrazolone
