摘要
目的:研究牛弥菜醇A对实验性糖尿病大鼠降糖作用及机制。方法:观察牛弥菜醇A对糖尿病大鼠的作用,给药14 d后,测定体重、空腹血糖(FBG)、血清胰岛素(INS)、血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)、血清总胆固醇(CHO)、甘油三酯(TG);取肝脏测定肝糖原含量;对胰腺做病理切片观察其形态学改变,并计算胸腺、胰腺、脾脏重指数。结果:与模型组相比,牛弥菜醇A(高、中剂量组)血糖值明显下降(P<0.01);高、中剂量组大鼠的血清SOD活性和INS水平明显增加(P<0.05),血清MDA含量、TG和TC水平明显降低(P<0.05),肝糖原含量明显提高(P<0.05);胸腺、胰腺、脾脏的重量指数明显升高(P<0.01或P<0.05);病理学观察发现,牛弥菜醇A各剂量组胰岛β细胞形态、结构与模型对照组比较,有不同程度的改善。结论:牛弥菜醇A降糖机制可能与改善机体对胰岛素的敏感性、清除自由基、促进肝糖原合成、提高机体抗氧化能力和增强免疫功能等作用有关。
Objective： To investigates the mechanism of hypooglycemic effect of conduritol A of stems of C-ymnema sylvestre. Method： Fourteen days later after administration, observation is taken on the change of these mice and rats ＇weight, the FBG, TG, CHO, SOD, MDA, INS, TNF in serum were also dectected with enzymology method and Radioimmuoassay mothod. Take the liver to determine the disposal of glucose. Take the pancreas to do the HE and immunohistoehemistrial staining, and show pancreas islet β- cell. Calulate thymus, pancreas, splenica index. Result： Compared with diabetic model mice, high and middosage of conduritol Aeould remarkably reduce fasted blood sugar in diabetic rats induced by alloxan （ P 〈 0. 01 ）. Significantly increase the level of serum insulin （ P 〈0. 05 ）. Activity of SOD was obviously inereasecd, and amount of MDA was obviously deerased （P 〈 0. 05 ）. The amount of conduritol A disposal of glucose was obviously increased （ P 〈 0. 05 ）. Significantly increase thymus, pancreas, splencia index （ P 〈 0. 01 or 0. 05 ） ; inhibited the atrophy of thymus, pancreas, splencias of the diabetic rats induced by alloxan. Compared with diabetic model group, ceil＇structure and form of conduritol A had been some way improved. The immunohistochemistry results showed that β- cells numbers of pancreas in each conduritol A group were more than those in the model group. Conclusion： Conduritol A could have an effect on regulating the metabolism of blood lipid, free-radical scavenging, enhancing the antioxidant ability, potentiating immune function. Promoting synthesis of hepatic to decrease fasted blood suger.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第24期2961-2965,共5页
China Journal of Chinese Materia Medica
基金
广西自然科学基金项目(桂科自0229044)
广西高校人才小高地建设创新团队资助计划
广西教育厅科研项目
广西中医学院重点科研项目
关键词
匙羹藤
牛弥菜醇A
降血糖作用
Gymnema sylvestre
conduritol A
hypoglycemic activity
