摘要
目的:建立一种简单快速测定诺氟沙星含量的一种新微分光度法。方法:将自行研制一套流动注射分析装置与双光束光度计相结合,实现微分光度的测定(ΔA)。基于氟哌酸(norfloxacin)在0.1 mol·L^(-1)盐酸介质中最大吸收波长为277 nm 的特点,直接用于诺氟沙星胶囊含量的测定。结果:分析速度190次·h^(-1),灵敏度是普通法的1.9倍,每次耗样量250μL,精密度为 RSD(%)=0.98(n=11)。结论:该方法操作简单,准确,灵敏,适合于诺氟沙星胶囊的质量控制,结果满意。
Objective： To develop a simple and quick method for the simultaneous determination of norfloxacin with FIA spectrophotometry. Methods：The determination of differential speetrophotometer method is achieved by the combination of set of self - fixed flow injection analysis equipment and a double beam light photometer. The suitable condition of the sample analysis is studied based on the property that a maximum absorption wavelength of 277 nm of fluperacid. In the medium of 0. 1 mol · L- 1 hydrochloric acid, it is directly applied to the determination of the content of Norfloxacin capsule. Result：The analytical rate is 190 times per hour. The sensitivity is 1.9 times of the common method. The quantity of sample consumed is 250 μL. The precision is RSD% = 0.98 （ n = 11 ）. Conclu- sion：The proposed method is simple, rapid and accurate which is suitable for quality control of compound capsules of norfloxaein.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第12期2129-2131,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
流动注射
微分光度法
诺氟沙星
双光束光度计
Flow injection
differential spectrophotometer
norfloxacin
double beam detector
