摘要
通过分析大肠杆菌的碳源代谢途径,利用基因敲除手段,以Escherichia coli MG1655为出发菌株,成功构建了琥珀酸好氧发酵生产工程菌E.coliQZ1111(MG1655ΔptsGΔpoxBΔptaΔiclRΔsdhA)。检测结果表明该菌株能以葡萄糖为碳源,在好氧发酵且不表达任何异源基因的条件下大量积累琥珀酸。摇瓶试验证明,琥珀酸发酵产量达到26.4g/L,乙酸盐作为唯一检测到的副产物产量为2.3g/L。二者浓度比达到11.5:1。
Based on carbon metabolic pathway analysis of Escherichia coli MG1655, an aerobic succinate fermentation platform was constructed by knocking out five genes （ptsG, poxB, pta, iclR and sdhA）, which was named E. coli QZ1111. Flask cultivation results showed that E. coli QZ1111 could accumulate succinate with a concentration of 26.4 g/L under aerobic conditions. The byproduct acetate was only 2.3 g/L. The production ratio of succinate and acetate reached 11.5：1.
出处
《生物工程学报》
CAS
CSCD
北大核心
2008年第12期2081-2085,共5页
Chinese Journal of Biotechnology
基金
国家高技术研究发展计划(863计划)(No.2006AA02Z218)
国家重点基础研究发展规划项目(973项目)(No.2007CB707803)资助~~
关键词
大肠杆菌
琥珀酸
代谢工程
Escherichia coli, succinic acid, metabolic engineering
