摘要
目的研究轮状病毒P[2]G3株在Vero细胞上的传代适应性,以确定其在Vero细胞上培养的最适条件。方法将P[2]G3株轮状病毒分别以0.01、0.05和0.1MOI接种于Vero细胞,观察细胞病变(CPE)情况,并检测病毒的感染性滴度,确定在Vero细胞上接种病毒的最适MOI。同时观察连续传15代后,各代次病毒的滴度及其基因核酸带型的变化。结果MOI为0.05时,接种病毒后出现CPE及收获时间均较早,病毒的感染性滴度最高,确定接种病毒的最适MOI为0.05。连续传15代,病毒滴度随传代次数的增加而上升,各代次病毒的基因组核酸带型基本一致,且与原代病毒相符。结论轮状病毒P[2]G3株经适应性培养后,可在Vero细胞上稳定传代15代。
Objective To study the adaptability of rotavirns P [2]G3 strain in Vero cells and optimize the condition for culture of the strain in Vero cells. Methods Rotavirus P[2]G3 strain was inoculated into Vero cells at MOIs of 0. 01, 0. 05 and 0. 1 respectively, then the CPE was observed and the infectious titer of virus was determined, based on which the MOI was optimized. The virus strain was subcuhured in Vero cells for 15 passages, and each passage was determined for titer and observed for genome banding pattern. Results The optimal MOI for inoculation of rotavirus P[2]G3 strain into Veto cells was 0. 05. During subculture, the virus titer increased with the increasing passage, and the genome banding pattern of various passages were basically consistent with that of original strain. Conclusion The rotavirus P[2]G3 strain after adaptation may be subcuhured stably for 15 passages in Vero cells.
出处
《中国生物制品学杂志》
CAS
CSCD
2008年第12期1078-1079,共2页
Chinese Journal of Biologicals
基金
国家863计划资助项目(2006AA02A211)
