摘要
维生素K3（Vitamin K3，VK3）是一种油溶性化舍物，不发荧光。L-半胱氨酸（L-cysteine，L-Cys）可将VK3还原为有荧光的甲萘酚，据此建立了一种测定药物制剂中VK3含量的新的荧光分光光度法。甲萘酚的激发波长和发射波长分别为389nm和520nm，荧光强度与VK3的浓度在3.2×10^-7－1.6×10^-5mol／L范围内呈良好的线性关系，相关系数为0.9994，检出限为1.2×10^-9mol／L，平均回收率为98.2％。方法用于VK3注射液的测定，结果令人满意。
Vitamin K3（VK3） is an oil-soluble vitamin and not naturally fluorescent but yields fluorescence when it is reduced to naphthohydroquinone by L-Cys, so a new method for determination of vitamin K3 was developed. All fluorescence measurements were performed at ,λex=389 nm and λem = 520 nm. The calibration curve had a linear range 3.2×10^-7-1.6×10^-5mol/L（R=0.9994） and a detection limit of 1.2×10^-9mol/L. The average recovery was 98.2%. The proposed method has been successfully applied to the determination of vitamin K3 in injections with satisfactory results.
出处
《分析科学学报》
CAS
CSCD
2008年第6期738-740,共3页
Journal of Analytical Science
